Effects of Ginkgo biloba extract (EGb 761) and quercetin on lipopolysaccharide-induced release of nitric oxide.

@article{Wadsworth2001EffectsOG,
  title={Effects of Ginkgo biloba extract (EGb 761) and quercetin on lipopolysaccharide-induced release of nitric oxide.},
  author={Teri L. Wadsworth and Dennis R. Koop},
  journal={Chemico-biological interactions},
  year={2001},
  volume={137 1},
  pages={
          43-58
        }
}
Administration of bacterial lipopolysaccharide (LPS) to laboratory animals and cultured macrophages is known to induce the production of nitric oxide (NO) from inducible nitric oxide synthase (iNOS). Here we show that pre-treatment with Ginkgo biloba extract (EGb 761) suppresses the in vivo production of NO (measured by the Griess reaction) after challenge with LPS. In order to begin to understand the mechanism of this inhibition, we evaluated in vitro effects of EGb 761 and its flavonoid… Expand
Preventive effect of Ginkgo biloba extract (GBB) on the lipopolysaccharide-induced expressions of inducible nitric oxide synthase and cyclooxygenase-2 via suppression of nuclear factor-kappaB in RAW 264.7 cells.
TLDR
GBB inhibits LPS-induced iNOS, COX-2 and TNF-alpha expressions through the down-regulation of NF-kappaB-DNA binding activity, suggesting that GBB is a more potent inhibitor of NO and PGE2 production than EGb 761. Expand
The effects of Ginkgo biloba extract on lipopolysaccharide-induced inflammation in vitro and in vivo.
TLDR
The present results indicate GBE suppresses the inflammation of EIU by blocking the iNOS protein expression and its anti-inflammatory effect on eye is comparable with the effect of prednisolone used in similar doses. Expand
Chlorella dichloromethane extract ameliorates NO production and iNOS expression through the down-regulation of NF kappa B activity mediated by suppressed oxidative stress in RAW 264.7 macrophages.
TLDR
CDE ameliorates NO production and iNOS expression through the down-regulation of NF kappa B activity, which may be mediated by attenuated oxidative stress in RAW 264.7 macrophages. Expand
Ginkgo biloba extract suppresses endotoxin-mediated monocyte activation by inhibiting nitric oxide- and tristetraprolin-mediated toll-like receptor 4 expression.
TLDR
Findings indicate that GBE could decrease the sensitivity of monocytes to LPS, and utilizing TTP to control TLR4 expression may be a promising approach for controlling systemic inflammation. Expand
Britanin suppresses LPS-induced nitric oxide, PGE2 and cytokine production via NF-κB and MAPK inactivation in RAW 264.7 cells.
TLDR
It is concluded that britanin may have potential for the treatment of inflammatory diseases through the down-regulation of MAP kinases and NF-κB mediated activation of macrophages. Expand
Inhibition of nitric oxide production from lipopolysaccharide-treated RAW 264.7 cells by synthetic flavones: Structure-activity relationship and action mechanism
TLDR
Of these flavonoids that were evaluated, 2′,3′,5,7-tet-rahydroxyflavone and 3′,4′, 5,7 -tetrahydroxflav one (luteolin) showed the strongest inhibition and may have a potential as new anti-inflammatory agents. Expand
Homocysteine stimulates inducible nitric oxide synthase expression in macrophages: Antagonizing effect of ginkgolides and bilobalide
TLDR
It is demonstrated that homocysteine, at pathophysiological concentrations, stimulates iNOS-mediated NO production in macrophages and EGb and its terpenoids can antagonize such stimulatory effect via antioxidation and attenuation of NF-κB activation. Expand
Effects of Indole-3-Carbinol and Flavonoids Administered Separately and in Combination on Nitric Oxide Production and iNOS Expression in Rats
TLDR
The results suggest that modifications of NO level in tissues by a natural compound cannot be predicted from data about its effects on NOS expression or activity, and stresses importance of direct measurements of NO in the tissues. Expand
Cedrela sinensis Leaves Suppress Oxidative Stress and Expressions of iNOS and COX-2 via MAPK Signaling Pathways in RAW 264.7 Cells
Overproduction of reactive oxygen species (ROS), including nitric oxide (NO), could be associated with the pathogenesis of various diseases such as cancer and chronic inflammation. Inducible nitricExpand
A novel antioxidant, octyl caffeate, suppression of LPS/IFN-gamma-induced inducible nitric oxide synthase gene expression in rat aortic smooth muscle cells.
TLDR
It is demonstrated that a novel potent antioxidant, octyl caffeate, significantly ameliorates circulatory failure of endotoxemia in vivo by a mechanism involving suppression of iNOS expression through inactivation of mitogen-activated protein kinases in RASMCs. Expand
...
1
2
3
4
5
...

References

SHOWING 1-10 OF 54 REFERENCES
Effects of Ginkgo biloba extract (EGb 761) and quercetin on lipopolysaccharide-induced signaling pathways involved in the release of tumor necrosis factor-α
TLDR
The results suggest that quercetin is unique in its ability to inhibit TNF-α transcription by inhibiting the phosphorylation and activation of JNK/SAPK and, therefore, suppressing AP-1-DNA binding [assessed by electrophoretic mobility shift analysis (EMSA). Expand
Ginkgo biloba extract (EGb 761): inhibitory effect on nitric oxide production in the macrophage cell line RAW 264.7.
TLDR
Findings indicate that not only does EGb 761 directly act as an NO scavenger but also that it inhibits NO production in LPS/IFN-gamma-activated macrophages by concomitant inhibition of induction ofiNOS mRNA and the enzyme activity of iNOS. Expand
(−)-Epigallocatechin-3-gallate Blocks the Induction of Nitric Oxide Synthase by Down-Regulating Lipopolysaccharide-Induced Activity of Transcription Factor Nuclear Factor-κB
TLDR
The results suggest that EGCG decreases the activity and protein levels of iNOS by reducing the expression ofiNOS mRNA and the reduction could occur through prevention of the binding of nuclear factor-kappaB to the iN OS promoter, thereby inhibiting the induction of i NOS transcription. Expand
Oroxylin A inhibition of lipopolysaccharide-induced iNOS and COX-2 gene expression via suppression of nuclear factor-kappaB activation.
TLDR
Results indicated that oroxylin A, an active component in Huang Qin, inhibited LPS-induced iNOS and COX-2 gene expression by blocking NF-kappaB activation, whereas emodin inhibition of L PS-inducediNOS expression may be mediated by a different transcription factor. Expand
Effects of the wine polyphenolics quercetin and resveratrol on pro-inflammatory cytokine expression in RAW 264.7 macrophages.
TLDR
The results do not support the view that wine antioxidants inhibit LPS-induced NF-KB activation but instead that they have a more selective action on genes activated by LPS. Expand
Peroxyl radical scavenging activity of Ginkgo biloba extract EGb 761.
TLDR
EGb 761 afforded protection against oxidative damage in all the systems the authors analyzed and is an efficient scavenger of peroxyl radicals, which supports the hypothesis of an antioxidant therapeutic action of EGb 7 61. Expand
Cardioprotective and anti-oxidant effects of the terpenoid constituents of Ginkgo biloba extract (EGb 761).
TLDR
Cardioprotective effects of therapeutically relevant doses of Ginkgo biloba extract and its terpenoid constituents appear to involve an inhibition of free radical formation rather than direct free radical scavenging. Expand
Pyrrolidine dithiocarbamate inhibits induction of nitric oxide synthase activity in rat alveolar macrophages.
TLDR
Findings suggest iNOS activity in macrophages treated with LPS + IFN gamma requires NF-kappa B activation, and Pyrrolidine dithiocarbamate added to cultured macrophage stimulated with lipopolysaccharide and interferon-gamma suggests this requirement. Expand
Effect of antioxidants, resveratrol, quercetin, and N‐acetylcysteine, on the functions of cultured rat hepatic stellate cells and kupffer cells
TLDR
NAC and these phenolic compounds may have therapeutic potential against liver injury by regulating functions of cultured rat hepatic stellate cells and Kupffer cells. Expand
Radicicol suppresses expression of inducible nitric-oxide synthase by blocking p38 kinase and nuclear factor-kappaB/Rel in lipopolysaccharide-stimulated macrophages.
TLDR
The inhibitory effects of radicicol on iNOS suggest that this potent antifungal agent may represent a useful anti-inflammatory agent. Expand
...
1
2
3
4
5
...