Effects of GUG and AUG initiation codons on the expression of lacZ in Escherichia coli.

Abstract

We have replaced the ribosomal binding site (RBS) of the lacZ gene of E. coli by those of the maturation (A) gene of phage MS2 and that of the tufA gene. Both RBSs contain a GUG initiation codon. The expression with the tufA RBS is at least 25-fold higher than with the phage RBS. Changing the GUG into AUG results in a 3-fold increase in expression in both… (More)

Topics

Figures and Tables

Sorry, we couldn't extract any figures or tables for this paper.