Effects of Ellman’s reagent and other thiol compounds on ion transport and ATPase activity in anaerobically grown Escherichia coli cells

@article{Poladyan2008EffectsOE,
  title={Effects of Ellman’s reagent and other thiol compounds on ion transport and ATPase activity in anaerobically grown Escherichia coli cells},
  author={Anna Poladyan and Karen Trchounian and L. Tadevosyan and Armen Trchounian},
  journal={Biochemistry (Moscow) Supplement Series A: Membrane and Cell Biology},
  year={2008},
  volume={2},
  pages={1-7}
}
It was found that modification of thiol (SH-) groups of membrane proteins by Ellman’s reagent (5,5′-dithiol-bis-(2-nitrobenzoic) acid) results in inhibition of proton efflux and K+ influx in anaerobically grown (pH 7.5) wild-type strains of Escherichia coli and causes disturbances in K+-dependent, N,N′-dicyclohexylcarbodiimide-sensitive ATPase activity and molecular hydrogen production. No such effects were observed after substitution of the cysteine residue in the b-subunit of F0 of proton… CONTINUE READING