Effects of Detergents on Activity, Thermostability and Aggregation of Two Alkalithermophilic Lipases from Thermosyntropha lipolytica

@inproceedings{Salameh2010EffectsOD,
  title={Effects of Detergents on Activity, Thermostability and Aggregation of Two Alkalithermophilic Lipases from Thermosyntropha lipolytica},
  author={Moh’d A. Salameh and Juergen Wiegel},
  booktitle={The open biochemistry journal},
  year={2010}
}

• Moh’d A. Salameh, Juergen Wiegel
• Published 2010 in The open biochemistry journal
• DOI:10.2174/1874091X01004010022

Thermosyntropha lipolytica DSM 11003, an anaerobic thermophilic lipolytic bacterium, produces the two alkalithermophilic lipases, LipA and LipB. Among all tested detergents, the two lipases were mostly affected by SDS when used at concentrations below its critical micelle concentration (CMC). In the absence of SDS, the v(max) of both LipA and LipB were 12.6 U.mg(-1) and 13.3 U.mg(-1) and K(0.5) were 1.8 mM and 1.65 mM, respectively at 96 degrees C and pH(opt) (25 masculineC)of 9.4-9.6. In the… CONTINUE READING

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