Effects of DNA sequence and histone-histone interactions on nucleosome placement.

@article{Shrader1990EffectsOD,
  title={Effects of DNA sequence and histone-histone interactions on nucleosome placement.},
  author={Theodore Shrader and D. M. Crothers},
  journal={Journal of molecular biology},
  year={1990},
  volume={216 1},
  pages={69-84}
}
Using competitive reconstitution, we have refined the parameters for the binding of histone octamers to artificial nucleosome-positioning sequences of the form: (A/T3nn(G/C)3nn. We find that the optimal period between flexible segments is approximately 10.1 base-pairs, supporting the view that the DNA on the nucleosome surface is overwound. The strongest… CONTINUE READING