Effects of DNA methyltransferase inhibitor RG108 on methylation in buffalo adult fibroblasts and subsequent embryonic development following somatic cell nuclear transfer.

@article{Sun2016EffectsOD,
  title={Effects of DNA methyltransferase inhibitor RG108 on methylation in buffalo adult fibroblasts and subsequent embryonic development following somatic cell nuclear transfer.},
  author={H. Sun and L. Meng and X. Zhao and J. Jiang and Q. Liu and D. Shi and F. Lu},
  journal={Genetics and molecular research : GMR},
  year={2016},
  volume={15 3}
}
  • H. Sun, L. Meng, +4 authors F. Lu
  • Published 2016
  • Biology, Medicine
  • Genetics and molecular research : GMR
Buffalo are characteristic livestock of the Guangxi Zhuang Autonomous Region of China, but their low reproductive capacity necessitates the use of somatic cell nuclear transfer (SCNT). We investigated the effects of RG108 on DNA methylation in buffalo adult fibroblasts, and on subsequent SCNT embryo development. RG108 treatment (0, 5, 10, 20, and 100 mM) had no effect on cell morphology, viability, or karyotype (2n = 48), and cell growth followed a typical "S" curve. Immunohistochemistry showed… Expand
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References

SHOWING 1-10 OF 27 REFERENCES
36 TREATMENT OF PORCINE FETAL FIBROBLASTS WITH DNA METHYLATION INHIBITORS OR HISTONE DEACETYLATION INHIBITORS IMPROVES DEVELOPMENT OF NUCLEAR TRANSFER EMBRYOS
TLDR
Treatment of donor cells with DNA methylation inhibitors or histone deacetylase inhibitors improved the subsequent blastocyst development of porcine reconstructed embryos even though combined treatment with both inhibitors had no beneficial effect. Expand
Significant improvement of mouse cloning technique by treatment with trichostatin A after somatic nuclear transfer.
TLDR
It is found that 5-50 nM TSA-treatment for 10 h following oocyte activation resulted in more efficient in vitro development of somatic cloned embryos to the blastocyst stage from 2- to 5-fold depending on the donor cells including tail tip cells, spleen cells, neural stem cells, and cumulus cells. Expand
Methylation and Acetylation Characteristics of Cloned Bovine Embryos from Donor Cells Treated with 5-aza-2′-Deoxycytidine1
TLDR
Embryos cloned from donor cells treated with a low dose of 5-aza-dC had higher levels of DNA methylation than embryos produced by in vitro fertilization, but they also hadHigher levels of histone acetylation, suggesting that this concentration of the drug was detrimental. Expand
Effect of DNMT inhibitor on bovine parthenogenetic embryo development.
TLDR
Results indicate that RG108, a DNMT inhibitor (DNMTi), inhibits the development of bovine parthenogenetic preimplantation embryos, suggesting that the DNMT is necessary for bovines parthenogenic preimplanatation embryo development. Expand
Somatic cell-induced hyperacetylation, but not hypomethylation, positively and reversibly affects the efficiency of in vitro cloned blastocyst production in cattle.
TLDR
The results indicate that somatic cells treated with TSA and NaB show better survival and recovery rates following the removal of these drugs, and H3K9 hyperacetylation, but not DNA hypomethylation (induced with AzC), favors cloning efficiency. Expand
Buffalos (Bubalus bubalis) Cloned by Nuclear Transfer of Somatic Cells1
TLDR
Results indicate that buffalo embryos derived from either fetal fibroblasts or granulosa cells can develop to the term of gestation and result in newborn calves. Expand
Increased pre-implantation development of cloned bovine embryos treated with 5-aza-2'-deoxycytidine and trichostatin A.
TLDR
Development of cloned bovine embryos was enhanced by 5-aza-dC and TSA; furthermore, the combination was more effective than either one alone. Expand
The effects of 5-aza-2'- deoxycytidine and trichostatin A on gene expression and DNA methylation status in cloned bovine blastocysts.
TLDR
It is likely that TSA may act synergistically with 5-aza-dC to exert such modifications in gene expression and DNA methylation, subsequently enhancing developmental potential (in vitro and full-term) of treated cloned embryos. Expand
Aberrant methylation of donor genome in cloned bovine embryos
TLDR
Evidence is provided that cloned bovine embryos may have impaired epigenetic reprogramming capabilities and highly aberrant methylation patterns in various genomic regions of cloned embryos, indicating that the developmental anomalies ofCloned embryos could be due to incomplete epigeneticReprogramming of donor genomic DNA. Expand
DNA methylation status of bovine blastocyst embryos obtained from various procedures.
TLDR
The findings suggest that the methylation level of the satellite I sequence at the BC stage is not related to the abnormalities of bovine embryos produced by NT-SC, and that the full-term developmental efficacy of these embryos were not different. Expand
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