Effects of DNA methyltransferase inhibitor RG108 on methylation in buffalo adult fibroblasts and subsequent embryonic development following somatic cell nuclear transfer.

  title={Effects of DNA methyltransferase inhibitor RG108 on methylation in buffalo adult fibroblasts and subsequent embryonic development following somatic cell nuclear transfer.},
  author={H. Sun and Lv Meng and X. Zhao and J R Jiang and Q. Liu and Deshun Shi and Fenghua Lu},
  journal={Genetics and molecular research : GMR},
  volume={15 3}
  • H. Sun, L. Meng, F. Lu
  • Published 2 September 2016
  • Biology
  • Genetics and molecular research : GMR
Buffalo are characteristic livestock of the Guangxi Zhuang Autonomous Region of China, but their low reproductive capacity necessitates the use of somatic cell nuclear transfer (SCNT). We investigated the effects of RG108 on DNA methylation in buffalo adult fibroblasts, and on subsequent SCNT embryo development. RG108 treatment (0, 5, 10, 20, and 100 mM) had no effect on cell morphology, viability, or karyotype (2n = 48), and cell growth followed a typical "S" curve. Immunohistochemistry showed… 

Figures and Tables from this paper

Optimizing treatment of DNA methyltransferases inhibitor RG108 on porcine fibroblasts for somatic cell nuclear transfer.

The results showed that RG108 treatment resulted in time-dependent decrease of genome-wide DNA methylation on fetal fibroblasts, which only happened after 72h treatment in the authors' experiments, and no interaction effect between treatment time and concentration.

Enhancement of epigenetic reprogramming status of porcine cloned embryos with zebularine, a DNA methyltransferase inhibitor

It is found that Zb could modify DNA methylation levels in the early stages of porcine SCNT embryos and promote their developmental competence.

Quisinostat treatment improves histone acetylation and developmental competence of porcine somatic cell nuclear transfer embryos

It is suggested that quisinostat can regulate gene expression and epigenetic modification, facilitating nuclear reprogramming, and subsequently improving the developmental competence of pig SCNT embryos and blastocyst quality.

Zebularine significantly improves the preimplantation development of ovine somatic cell nuclear transfer embryos.

The results indicate that, in an ovine model, zebularine decreases overall DNA methylation levels in donor cumulus cells and reconstructed embryos, downregulates theDNA methylation profile in the promoter region of pluripotency genes in donor cells and ultimately elevates the expression of plurine genes in the reconstruct embryos, which can lead to improved development of SCNT embryos.

Dynamic Methylation Changes of DNA and H3K4 by RG108 Improve Epigenetic Reprogramming of Somatic Cell Nuclear Transfer Embryos in Pigs

Dynamic changes in DNA methylation caused by RG108 result in dynamic alterations in the patterns of H3K4me3, H3 k9Ac and histone H3 lysine 9 trimethylation, which leads to the activation of embryonic genome and epigenetic modification enzymes associated with H3k4 methylation, and contributes to reconstructing normal epigenetic modifications and improving the developmental efficiency of porcine SCNT embryos.

DNA methylation studies in cattle

Overall, the research is directed towards the identification of single methylation markers or their combinations which may be useful in the selection and breeding of animals to ensure cattle improvement.

siRNA inhibition and not chemical inhibition of Suv39h1/2 enhances pre-implantation embryonic development of bovine somatic cell nuclear transfer embryos

H3K9me3 is demonstrated as an epigenetic barrier in the reprogramming process mediated by SCNT in bovine species, a finding which supports the role of H3K 9me3 as a reprograming barrier in mammalian species.

Current status of assisted reproductive technologies in buffaloes.

Buffaloes are raised by small farm holders primarily as source of draft power owing to its resistance to hot climate, disease, and stress conditions. Over the years, transformation of these animals

The OsmiR396–OsGRF8–OsF3H‐flavonoid pathway mediates resistance to the brown planthopper in rice (Oryza sativa)

A new BPH resistance mechanism mediated by the OsmiR396–OsGRF8–OsF3H–flavonoid pathway is revealed and potential applications of miRNAs in B PH resistance breeding are suggested.

Reproduction Advances in Buffaloes

The buffalo (Bubalus bubalis) is a prime livestock species reared for milk, meat, skin, and draught power in Asian and Mediterranean countries and the research areas which need further scientific inputs include molecular cataloguing and conservation of native breeds.



Significant improvement of mouse cloning technique by treatment with trichostatin A after somatic nuclear transfer.

Methylation and Acetylation Characteristics of Cloned Bovine Embryos from Donor Cells Treated with 5-aza-2′-Deoxycytidine1

Embryos cloned from donor cells treated with a low dose of 5-aza-dC had higher levels of DNA methylation than embryos produced by in vitro fertilization, but they also hadHigher levels of histone acetylation, suggesting that this concentration of the drug was detrimental.

Effect of DNMT inhibitor on bovine parthenogenetic embryo development.

Somatic cell-induced hyperacetylation, but not hypomethylation, positively and reversibly affects the efficiency of in vitro cloned blastocyst production in cattle.

The results indicate that somatic cells treated with TSA and NaB show better survival and recovery rates following the removal of these drugs, and H3K9 hyperacetylation, but not DNA hypomethylation (induced with AzC), favors cloning efficiency.

Anti-tumoral effect of the non-nucleoside DNMT inhibitor RG108 in human prostate cancer cells.

RG108 is an effective tumor growth suppressor in most PCa cell lines tested and is likely mediated by reversion of aberrant DNA methylation affecting cancer related-genes epigenetically silenced in PCa.

Identification of transgenic cloned dairy goats harboring human lactoferrin and methylation status of the imprinted gene IGF2R in their lungs.

Results showed that the IGF2R gene in the lungs of the dead cloned kids showed abnormal hypermethylation and higher mRNA expression levels than the control, indicating that aberrant DNA methylation reprogramming is one of the important factors in the death of transgenic cloned animals.

Active demethylation of the paternal genome in the mouse zygote

DNA methylation variation in cloned mice

Cloned animals are by no means perfect copies of the original animals as far as the methylation status of genomic DNA is concerned, and individual cloned animals have different methylation aberrations.