Effects of DNA methyltransferase inhibitor RG108 on methylation in buffalo adult fibroblasts and subsequent embryonic development following somatic cell nuclear transfer.

  title={Effects of DNA methyltransferase inhibitor RG108 on methylation in buffalo adult fibroblasts and subsequent embryonic development following somatic cell nuclear transfer.},
  author={H. Sun and L. Meng and X. Zhao and J. Jiang and Q. Liu and D. Shi and F. Lu},
  journal={Genetics and molecular research : GMR},
  volume={15 3}
  • H. Sun, L. Meng, +4 authors F. Lu
  • Published 2016
  • Biology, Medicine
  • Genetics and molecular research : GMR
Buffalo are characteristic livestock of the Guangxi Zhuang Autonomous Region of China, but their low reproductive capacity necessitates the use of somatic cell nuclear transfer (SCNT). We investigated the effects of RG108 on DNA methylation in buffalo adult fibroblasts, and on subsequent SCNT embryo development. RG108 treatment (0, 5, 10, 20, and 100 mM) had no effect on cell morphology, viability, or karyotype (2n = 48), and cell growth followed a typical "S" curve. Immunohistochemistry showed… Expand
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