Effects of CTGF/Hcs24, a product of a hypertrophic chondrocyte-specific gene, on the proliferation and differentiation of chondrocytes in culture.

@article{Nakanishi2000EffectsOC,
  title={Effects of CTGF/Hcs24, a product of a hypertrophic chondrocyte-specific gene, on the proliferation and differentiation of chondrocytes in culture.},
  author={T. Nakanishi and T. Nishida and T. Shimo and K. Kobayashi and T. Kubo and T. Tamatani and K. Tezuka and M. Takigawa},
  journal={Endocrinology},
  year={2000},
  volume={141 1},
  pages={
          264-73
        }
}
Recently, we cloned a messenger RNA (mRNA) predominantly expressed in chondrocytes from a human chondrosarcoma-derived chondrocytic cell line, HCS-2/8, by differential display PCR and found that its gene, named hcs24, was identical with that of connective tissue growth factor (CTGF). Here we investigated CTGF/Hcs24 function in the chondrocytic cell line HCS-2/8 and rabbit growth cartilage (RGC) cells. HCS-2/8 cells transfected with recombinant adenoviruses that generate CTGF/Hcs24 sense RNA… Expand
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TLDR
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References

SHOWING 1-10 OF 68 REFERENCES
Cloning of a mRNA preferentially expressed in chondrocytes by differential display-PCR from a human chondrocytic cell line that is identical with connective tissue growth factor (CTGF) mRNA.
TLDR
Findings suggest that CTGF participates in endochondral ossification and is expressed only in the hypertrophic chondrocytes of costal cartilage and the vertebral column in embryonic mice. Expand
Inhibition of endogenous expression of connective tissue growth factor by its antisense oligonucleotide and antisense RNA suppresses proliferation and migration of vascular endothelial cells.
TLDR
Findings suggest that endogenous CTGF expression is involved in the proliferation and migration of BAE cells, which were stably transfected with expression vectors that generate the antisense RNA of CTGF cDNA. Expand
Demonstration of receptors specific for connective tissue growth factor on a human chondrocytic cell line (HCS-2/8).
TLDR
The presence of receptors specific for connective tissue growth factor (CTGF) was demonstrated on a human chondrosarcoma-derived chondrocytic cell line, HCS-2/8, and findings suggest that CTGF directly binds to specific receptor molecules on HCS/8 cells. Expand
Purification of an angiogenesis inhibitor from culture medium conditioned by a human chondrosarcoma-derived chondrocytic cell line, HCS-2/8.
TLDR
The findings clearly showed for the first time that chondrocytes themselves produce a potent inhibitor of angiogenesis, which is also an inhibitor of gelatinase, and indicate that hCHIAMP is a TIMP-2-like molecule. Expand
Establishment from a human chondrosarcoma of a new immortal cell line with high tumorigenicity in vivo, which is able to form proteoglycan‐rich cartilage‐like nodules and to respond to insulin in vitro
TLDR
Inoculation of HCS-2/A cells into athymic mice resulted in the formation of chondrosarcomas that grew faster than those arising from HCS‐2/8 cells, and Immunostaining revealed that H CS‐2 /A cells produced type‐11 collagen but not type‐1 collagen. Expand
Establishment of a clonal human chondrosarcoma cell line with cartilage phenotypes.
TLDR
A clonal cell line with cartilage phenotypes and tumorigenicity during more than 3 years in culture was established from a human chondrosarcoma, which consisted of slightly elongated polygonal cells, which proliferated with a doubling time of 3.5 days and formed nodules, which showed metachromasia when stained with toluidine blue. Expand
Fisp12/Mouse Connective Tissue Growth Factor Mediates Endothelial Cell Adhesion and Migration through Integrin αvβ3, Promotes Endothelial Cell Survival, and Induces Angiogenesis In Vivo
TLDR
It is suggested that Fisp12/mCTGF and CYR61 comprise prototypes of a new family of angiogenic regulators that function, at least in part, through integrin-αvβ3-dependent pathways. Expand
CYR61, a product of a growth factor-inducible immediate early gene, promotes angiogenesis and tumor growth.
TLDR
It is shown that purified CYR61 stimulates directed migration of human microvascular endothelial cells in culture through an alphaV beta3-dependent pathway and induces neovascularization in rat corneas and potential roles for CYR 61 in physiologic and pathologic neov vascularization are suggested. Expand
Cyr61, product of a growth factor-inducible immediate-early gene, regulates chondrogenesis in mouse limb bud mesenchymal cells.
TLDR
It is demonstrated that the Cyr61 protein promotes chondrogenesis in micromass cultures of limb bud mesenchymal cells in vitro and is likely to play a similar role in vivo and identify Cyr61 as a novel player in chONDrogenesis that contributes to the development of the mammalian embryonic skeleton. Expand
Insulin-like growth factors I and II are autocrine factors in stimulating proteoglycan synthesis, a marker of differentiated chondrocytes, acting through their respective receptors on a clonal human chondrosarcoma-derived chondrocyte cell line, HCS-2/8.
TLDR
Findings indicate that IGF-I and IGF-II act as autocrine differentiation factors for this chondrosarcoma-derived chondrocytic permanent cell line, HCS-2/8, mainly via respective receptors. Expand
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