Effects of BSO and DEM on thiol-level and radiosensitivity in HeLa cells.

  title={Effects of BSO and DEM on thiol-level and radiosensitivity in HeLa cells.},
  author={O. Vos and Govert P van der Schans and W S Roos-Verhey},
  journal={International journal of radiation oncology, biology, physics},
  volume={10 8},

The influence of oxygen on the induction of radiation damage in DNA in mammalian cells after sensitization by intracellular glutathione depletion.

The widely accepted idea that intracellular SH-compounds compete with oxygen and other electron-affinic radiosensitizers with respect to reaction with radiation-induced damage is strengthened, thus preventing the fixation of DNA damages by oxygen.

Reduction of intracellular glutathione content and radiosensitivity.

The intracellular glutathione (GSH) content of HeLa, CHO and V79 cells was reduced by incubating the cells in growth medium containing buthionine sulphoximine or diethyl maleate (DEM), and no effect was found on post-irradiation repair of ssb and dsb.

The influence of cellular glutathione content on cell survival following photodynamic treatment in vitro.

Cell survival of GSH-deficient human fibroblasts was decreased following PDT and gamma irradiation when compared to their normal counterparts, and in both modalities the difference was mainly due to a reduction in the Dq's, while the Do's were only slightly affected.

Effect of glutathione depletion on the cytotoxicity of xenobiotics and induction of single-strand DNA breaks by ionizing radiation in isolated hamster round spermatids.

The present results indicate that cellular GSH has an important function in the defence mechanisms of round spermatids against peroxides, electrophilic xenobiotics and radiation-induced DNA damage.

Glutathione manipulation and the radiosensitivity of human tumour and fibroblast cell lines.

The role of glutathione (GSH) in determining radiation response in five human tumour and one human fibroblast cell line was studied and compared with clonogenic survival following gamma-irradiation, with no relationship between GSH concentration and aerobic radiosensitivity.

The effect of GSH depletion on thermal radiosensitization.

Erastin, a ferroptosis-inducing agent, sensitized cancer cells to X-ray irradiation via glutathione starvation in vitro and in vivo

The radiosensitizing effect of erastin on two adenocarcinoma cell lines and the tumor xenograft model accompanied by glutathione depletion is demonstrated, indicating that ferroptosis inducers that reduce glutathion concentration could be applied as a novel cancer therapy in combination with radiotherapy.

Some aspects of glutathione metabolism in ataxia-telangiectasia fibroblasts.

  • S. Dean
  • Biology, Medicine
    International journal of radiation biology and related studies in physics, chemistry, and medicine
  • 1987
There was some variation in GST activity among the four cell lines but deficiency in this enzyme cannot be associated with radiosensitivity in A-T, and this small deficiency could be due to chance and is unlikely to be responsible for the radiosensitive phenotype of A- T.



The role of thiols in cellular response to radiation and drugs.

An altered thiol model is proposed which includes a mechanism for thiol involvement in the aerobic radiation response of cells and involves both thiol-linked hydrogen donation to oxygen radical adducts to produce hydroperoxides followed by a GSH peroxidase-catalyzed reduction of the hydroperoxide to intermediates entering into metabolic pathways to produce the original molecule.

Lack of oxygen effect in glutathione-deficient human cells in culture.

The frequency of X-ray-induced DNA breaks was determined in human cell lines which are deficient in glutathione synthetase and have a greatly reduced glutathion content, and the dose-effect relationship for the induction of breaks when radiation exposure was made in argon, was similar to that found when exposure wasmade in air.

Cellular glutathione is a key to the oxygen effect in radiation damage

It is reported here that one major cellular sulphydryl constituent, glutathione (GSH), is apparently the major component in the interaction between radiation products and the cell, for cells unable to synthesise glutATHione cannot be protected against killing by ionising radiation by reduction of the external oxygen concentration.

Induction and repair of single-strand DNA breaks after X-irradiation of human fibroblasts deficient in glutathione.

  • M. EdgrenL. RévészA. Larsson
  • Biology, Medicine
    International journal of radiation biology and related studies in physics, chemistry, and medicine
  • 1981
The data are interpreted as indicating that the repair mechanism for oxically and anoxically induced single-strand breaks is qualitatively different, and requires glutathione in the former case.

Glutathione export by human lymphoid cells: depletion of glutathione by inhibition of its synthesis decreases export and increases sensitivity to irradiation.

  • J. DethmersA. Meister
  • Biology
    Proceedings of the National Academy of Sciences of the United States of America
  • 1981
Depletion of glutathione by use of buthionine sulfoximine has advantages over other reagents and therefore has potential usefulness in sensitizing cells to the effects of radiation and to therapeutic agents that are detoxified by reactions involving glutATHione.

Oxygen enhancement of radiation induced lethality is greatly reduced in glutathione deficient human fibroblasts.

The in vitro clonogenic survival of human fibroblasts with a genetically defined glutathione (GSH) deficiency was studied after irradiation with X-rays in oxygen or in oxygen free argon to support the theory according to which oxygen and GSH compete for radiation induced radicals in key molecules.

Induction and repair of DNA damage in normal and ataxia-telangiectasia skin fibroblasts treated with neocarzinostatin.

NCS is an appropriate agent for studying the kinetics of rejoining strand breaks, due to its rapid action in the cells; this action, which is completed within 2--4 min, was studied by monitoring strand break induction, inhibition of DNA synthesis and decrease in cellular survival.

Radiosensitization of hypoxic tumor cells by depletion of intracellular glutathione.

Depletion of glutathione in Chinese hamster ovary cells in vitro by diethyl maleate resulted in enhancement of the effect of x-rays on cell survival under hypoxic conditions but not under oxygenated conditions, suggesting that the effectiveness of misonidazole in cancer radiotherapy may be improved by combining it with drugs that deplete intracellular glutATHione.

DNA strand break and rejoining in cultured human fibroblasts exposed to fast neutrons or gamma rays.

The comparison of neutrons and gamma-rays in the induction of DNA breaks did not explain the elevated r.b.e. on high LET radiation; however, a study of the variation in the spectrum of lesions induced by different radiation sources will probably contribute to the clarification of the relative importance of other radio products.

X-ray induced DNA double strand break production and repair in mammalian cells as measured by neutral filter elution.

The introduction of double strand cuts by HpA I restriction endonuclease in DNA lysed on filters results in a rapid rate of elution under neutral conditions, implying that the method can detect double strand breaks if they exist in the DNA.