Effects of Asp-179 mutations in TEMpUC19 beta-lactamase on susceptibility to beta-lactams.

@article{Vakulenko1995EffectsOA,
  title={Effects of Asp-179 mutations in TEMpUC19 beta-lactamase on susceptibility to beta-lactams.},
  author={Sergei B. Vakulenko and Marta Toth and Pascale Veronique Taibi and Shahriar Mobashery and Stephen A. Lerner},
  journal={Antimicrobial agents and chemotherapy},
  year={1995},
  volume={39 8},
  pages={
          1878-80
        }
}
To examine the effect of disruption of the salt bridge (between Arg-164 and Asp-179 [numbering of Ambler et al. (Biochem J. 267:269-272, 1991)]) that anchors the conserved omega-loop in class A beta-lactamases, we obtained mutant enzymes with each of the 19 other amino acid residues replacing Asp-179 in the TEM beta-lactamase encoded by pUC19 and studied the level of resistance to various beta-lactams conferred by each enzyme. All mutations of Asp-179 compromised the level of resistance to… CONTINUE READING
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