Effects of Alfalfa Saponins on In Vitro Physiological Activity of Soil and Rhizosphere Bacteria

Abstract

/a vitro eft'ects of purified alla.lta lMedicago salitq. L.) saponins (s(ryasaponin I, medicagenic acid IMA-3,28Ni1, tr,tR-.rg,u, 28Na. and MA--1.28g1u) on rlrizospherc hacteiia cell suspensions r,,,.-cr r investigated. Triphr:rryltetrazolirrrn chluride (TTC)-dehvclrorcnase nnd f l r rorescein d iacctatc (FDA) hydro ly t ic act iv i t ies wcre-s l ro ie lv inh ihitcd (90-95%) try M,4-.3.28Na nn1 1qd-.?gtu.28Na in Bncilli i tlruringlensls strains (HD-2 and \JZA\4\. In Curtobsclerium flacunraf.tciens (JM10l1), TTC and FDA activitids were reduced only tiy trtA-3,28trta, while little or no etl'ects were observed on the gram-negative .strains Pselcloutonts lluorescens (RA-2) and Agrobicterimntumefaciens (A-136). Soyasaponin I decreased FDA hydrolysis in HD-2 and JMl011, hut incrcased FDA activity by 2-tbld in IiA-2. MA-lelu,28Nr incrersctl protein e;,udation or leakage by .5 to l0-lbld in ail slrlirrs, antr MA increased leakage in all strains except RA-2. Soyasaponin in-

Cite this paper

@inproceedings{Hoagland2006EffectsOA, title={Effects of Alfalfa Saponins on In Vitro Physiological Activity of Soil and Rhizosphere Bacteria}, author={Robert Edward Hoagland and Robert M. Zablotowicz and Wieslaw A. Oleszek}, year={2006} }