Effects of 4‐hexylresorcinol on the phenoloxidase from Hyphantria cunea (Lepidoptera: Arctiidae): In vivo and in vitro studies

@article{Sharifi2015EffectsO4,
  title={Effects of 4‐hexylresorcinol on the phenoloxidase from Hyphantria cunea (Lepidoptera: Arctiidae): In vivo and in vitro studies},
  author={Mahboobeh Sharifi and Mohammad Ghadamyari and Reza Hasan Sajedi and Nosrat O. Mahmoodi},
  journal={Insect Science},
  year={2015},
  volume={22}
}
Insecticidal effects of 4‐hexylresorcinol, a phenoloxidase (PO) inhibitor, were determined on Hyphantria cunea (Drury) under laboratory conditions. The LC50 for the 15‐d‐old larvae was estimated to be 2.95 g/L after 96 h exposure. The activities of glutathione S‐transferase (GST) and PO showed a decrease in larvae treated with 4‐hexylresorcinol, and the IC50 of GST and PO were estimated to be 0.8 and 0.43 g/L, respectively, 24 h after treatment. The PO from the hemolymph of fall webworm was… 
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References

SHOWING 1-10 OF 46 REFERENCES
Enzymatic properties of phenoloxidase from Pieris rapae (Lepidoptera) larvae
The kinetic parameters of partially purified phenoloxidase (PO, EC. 1.14.18.1) from the 5th instar larvae of Pieris rapae (Lepidoptera) were determined, using L‐3, 4‐dihydroxyphenylalanine (L‐DOPA)
Insecticidal effects of 4-hexylresorcinol on the lesser mulberry snout moth, Glyphodes pyloalis Walker
TLDR
4-hexylresorcinol is bioassayed against the Glyphodes pyloalis Walker, an important pest of mulberry trees in north of Iran and this pest interferes with the sericulture by feeding on mulberry leaves.
Purification and characterization of phenoloxidase from crab Charybdis japonica.
The effects of luteolin on phenoloxidase and the growth of Spodoptera exigua (Hübner) larvae (Lepidoptera: Noctuidae)
TLDR
The effects of luteolin on phenoloxidase (PO) from Spodoptera exigua larvae were investigated with the use of a microtitration assay and the compound was found to be a reversible competitive inhibitor with a K i of 13.11 mmol/l.
Isolation and characterization of the cDNA encoding the prophenoloxidase of fall webworm, hyphantria cunea.
Purification and characterization of hemolymph prophenoloxidase from Ostrinia furnacalis (Lepidoptera: Pyralidae) larvae.
Inhibitory Effects of Hexylresorcinol and Dodecylresorcinol on Mushroom (Agaricus bisporus) Tyrosinase
TLDR
The results show that hexylresorcinol and dodecylresOrcinol can inhibit both monophenol enzyme and diphenolase activity of the enzyme, and the steady-state activity of this enzyme decreased sharply.
...
...