Effects of 15-deoxy-delta12,14-prostaglandin J2 on cell proliferation and apoptosis in ECV304 endothelial cells.

@article{Dong2004EffectsO1,
  title={Effects of 15-deoxy-delta12,14-prostaglandin J2 on cell proliferation and apoptosis in ECV304 endothelial cells.},
  author={Yu-gang Dong and Dan-dan Chen and Jian-gui He and Yong-Yuan Guan},
  journal={Acta pharmacologica Sinica},
  year={2004},
  volume={25 1},
  pages={47-53}
}
AIM To investigate the effects of 15-deoxy-delta12,14-prostaglandin J2 (15d-PGJ2) on cell proliferation and apoptosis in ECV304 endothelial cells and related molecular mechanism. METHODS MTT, Hoechst33258, TUNEL, Flow cytometry, DNA ladder, RT-PCR, Western blot, and electrophoretic mobility shift assay (EMSA) analysis were employed. RESULTS The 15d-PGJ2 induced apoptosis in ECV304 endothelial cells in a dose-dependent manner (the percentage of apoptosis was enhanced from 10.0 %+/-1.3 % to… CONTINUE READING