Effects of 12-O-tetradecanoylphorbol-13-acetate on estrogen receptor activity in MCF-7 cells.

  title={Effects of 12-O-tetradecanoylphorbol-13-acetate on estrogen receptor activity in MCF-7 cells.},
  author={Mary Beth Martin and Pilar Garcia-Morales and Adriana Stoica and Harrison B Solomon and Michael Pierce and David B. Katz and Shuping Zhang and Mark Danielsen and Miguel Saceda},
  journal={The Journal of biological chemistry},
  volume={270 42},
The effects of long term treatment with 12-O-tetradecanoylphorbol-13-acetate (TPA) on estrogen receptor (ER) expression in the human breast cancer cell line, MCF-7, were studied. This study demonstrates that treatment of cells with the phorbol ester blocked estrogen receptor activity. Treatment of cells with 100 nM TPA resulted in an 80% decrease in the level of ER protein and a parallel decrease in ER mRNA and binding capacity. Following removal of TPA from the medium, the level of ER protein… CONTINUE READING


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