Effects of α and β recombinant FSH (Gonal-F, Puregon) and progesterone upon human endometrial cell proliferation in-vitro: a preliminary study

  title={Effects of $\alpha$ and $\beta$ recombinant FSH (Gonal-F, Puregon) and progesterone upon human endometrial cell proliferation in-vitro: a preliminary study},
  author={Chi‐Chen Chang and Yao Yuan Hsieh and Kung‐Hao Hsu and Chih‐Sheng Lin},
  journal={Gynecological Endocrinology},
  pages={110 - 116}
Endometrial proliferation or regeneration during menstrual cycle is regulated by sexual hormones. However, the effect of gonadotrophins on the endometrial cell growth remains obscure. Herein, we aimed to investigate the effects of r-FSH (Gonal-F, Puregon) and progesterone on the proliferation of human endometrial cells in-vitro. According as gonadotrophin concentrations, the follicular-phase endometrial cells were divided into six groups: (1) 0 (controls), (2) 1; (3) 10; (4) 100; (5) 1000; (6… 
Effects of Steroid Hormones on Epithelial and Stromal Cells Culture and Proliferation of Laying Hens , In vitro
The results showed that endometrial epithelial and stromal cells were successfully isolated and cultured, both of which began to enter the logarithmic phase of growth after 24 h and reached the plateau phase at 7 days after inoculation, which can suppose that steroid hormones could positively effect on the proliferation ofendometrial cells of laying hens.
Follicle-stimulating hormone administration affects amino acid metabolism in mammalian oocytes†
It is proposed that the effect of FSH treatment on amino acid uptake is influenced by FSH receptor with the effect on oocyte metabolism and physiology.
Tissue expression and serum levels of periostin during pregnancy: a new biomarker of embryo-endometrial cross talk at implantation.
Global Gene Expression Profiling of Proliferative Phase Endometrium Reveals Distinct Functional Subdivisions
A unique gene expression signature at distinct time points within the proliferative phase is demonstrated, demonstrating the onset of expression of genes required for endometrial receptivity and a dampening of estrogen responsiveness.
An update on the progress of transcriptomic profiles of human endometrial receptivity†
Abstract Despite advances in our understanding of fertility, implantation failure remains a significant problem for both spontaneous and assisted pregnancies. Most research efforts concerning the
Biomarqueurs de la réceptivité endométriale humaine : du fondamental aux applications cliniques
La receptivite endometriale des patientes sous traitements hormonaux substitutifs differait de celles en cycle naturel, caracterisee par une alteration des voies de signalisation mediee par les recepteurs aux œstrogenes, des membres of the famille VEGF and des integrines.
Endometrial Receptivity Profile in Patients with Premature Progesterone Elevation on the Day of hCG Administration
Endometrial gene expression shift from the prereceptive to the receptive stage was altered in patients with high serum progesterone level on the day of hCG administration, suggesting accelerated endometrial maturation during the periovulation period.


Effect of gonadotropins on human endometrial stromal cell proliferation in vitro
It is demonstrated that FSH and hMG induced significant inhibition in [3H]-thymidine uptake at all concentrations, respectively, which implies that gonadotropins at the concentrations studied inhibit the proliferation of human endometrial stromal cells, at least, in short-term culture in vitro.
Progesterone, but not estrogen, stimulates vessel maturation in the mouse endometrium.
Results show that progesterone, but not estrogen, stimulates vessel maturation in the mouse endometrium, illustrating the relevancy of the mouse model for understanding endometrial vascular remodeling during the menstrual cycle and in response to the clinically important progester one receptor antagonist RU486.
Recombinant follicle stimulating hormone in in-vitro fertilization treatment-clinical experience with follitropin alpha and follitropin beta.
Gonal-F and Puregon may be equally suitable for use in ovarian stimulation for IVF, and it was not possible to find significant differences in the IVF programme with regard to stimulation outcome.
Sequential hormonal supplementation with vaginal estradiol and progesterone gel corrects the effect of clomiphene on the endometrium in oligo-ovulatory women.
The addition of vaginal E2 and progesterone to CC ovulation induction regimens normalizes the alterations in endometrial morphology and may improveendometrial receptivity in CC cycles and ultimately yield higher pregnancy rates.
Homologous down-regulation of luteinizing hormone/chorionic gonadotropin receptors by increasing the degradation of receptor transcripts in human uterine endometrial stromal cells.
The homologous down-regulation observed in the present study may potentially explain low endometrial receptor levels in the postmenopausal human endometrium.
Effect of angiogenesis inhibitors on oestrogen-mediated endometrial endothelial cell proliferation in the ovariectomized mouse.
It is demonstrated that endometrial angiogenesis after acute oestrogen treatment is primarily mediated by VEGF, but that under the influence of combined oestrogens and progesterone, FGF and PDGF are also probably involved.
More basic forms of both human follicle-stimulating hormone and luteinizing hormone in serum at midcycle compared with the follicular or luteal phase.
  • L. WideO. Bakos
  • Medicine, Biology
    The Journal of clinical endocrinology and metabolism
  • 1993
It is concluded that at least 20-30 isoforms of both FSH and LH circulate in blood during the menstrual cycle, more basic than in the follicular or luteal phase, which is most likely due to a selective secretion from the pituitary of more basic forms of F SH and LH at midcycle.
Aberrant expression of angiopoietins-1 and -2 and vascular endothelial growth factor-A in peri-implantation endometrium after gonadotrophin stimulation.
The decreasedAng-1 concentration and Ang-1/Ang-2 ratio and the increased Ang-2 concentration, with the increased vWF concentration and blood vessel density, in stimulated cycles suggests advanced endometrial angiogenesis after gonadotrophin-stimulation.