Effectors of the stringent response target the active site of Escherichia coli adenylosuccinate synthetase.

@article{Hou1999EffectorsOT,
  title={Effectors of the stringent response target the active site of Escherichia coli adenylosuccinate synthetase.},
  author={Zhenglin Hou and Michael Cashel and Herbert J. Fromm and Richard B. Honzatko},
  journal={The Journal of biological chemistry},
  year={1999},
  volume={274 25},
  pages={17505-10}
}
Guanosine 5'-diphosphate 3'-diphosphate (ppGpp), a pleiotropic effector of the stringent response, potently inhibits adenylosuccinate synthetase from Escherichia coli as an allosteric effector and/or as a competitive inhibitor with respect to GTP. Crystals of the synthetase grown in the presence of IMP, hadacidin, NO3-, and Mg2+, then soaked with ppGpp, reveal electron density at the GTP pocket which is consistent with guanosine 5'-diphosphate 2':3'-cyclic monophosphate. Unlike ligand complexes… CONTINUE READING