Effector kinase coupling enables high-throughput screens for direct HIV-1 Nef antagonists with antiretroviral activity.


HIV-1 Nef, a critical AIDS progression factor, represents an important target protein for antiretroviral drug discovery. Because Nef lacks intrinsic enzymatic activity, we developed an assay that couples Nef to the activation of Hck, a Src family member and Nef effector protein. Using this assay, we screened a large, diverse chemical library and identified… (More)
DOI: 10.1016/j.chembiol.2012.11.005


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