Effectiveness and stability of heterologous proteins expressed in plants by Turnip mosaic virus vector at five different insertion sites.

@article{Chen2007EffectivenessAS,
  title={Effectiveness and stability of heterologous proteins expressed in plants by Turnip mosaic virus vector at five different insertion sites.},
  author={Chin-Chih Chen and Tsung-Chi Chen and Joseph A. J. Raja and Claudia Chang and Li-wen Chen and Shih-Shun Lin and Shyi-dong Yeh},
  journal={Virus research},
  year={2007},
  volume={130 1-2},
  pages={
          210-27
        }
}
The N-terminal (NT) regions of particular protein-coding sequences are generally used for in-frame insertion of heterologous open reading frames (ORFs) in potyviral vectors for protein expression in plants. An infectious cDNA clone of Turnip mosaic virus (TuMV) isolate YC5 was engineered at the generally used NT regions of HC-Pro and CP, and other possibly permissive sites to investigate their effectiveness to express the GFP (jellyfish green fluorescent protein) and Der p 5 (allergen from the… CONTINUE READING

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