Effective selection system for generating marker-free transgenic plants independent of sexual crossing

@article{Sugita1999EffectiveSS,
  title={Effective selection system for generating marker-free transgenic plants independent of sexual crossing},
  author={Koichi Sugita and Eisuke Matsunaga and Hiroyasu Ebinuma},
  journal={Plant Cell Reports},
  year={1999},
  volume={18},
  pages={941-947}
}
 In a previous report, a novel selection protocol termed "the MAT-vector system" for generating marker-free transgenic plants (MFTPs) was presented. The first stage of the system is visual selection of morphologically abnormal transgenic shoots, ipt-shooty, that have lost apical dominance and rooting ability. The second stage involves elimination of the ipt gene and the appearance of MFTPs free of ipt gene influence. The present report describes a practical MAT-vector in which removal of the… CONTINUE READING

Citations

Publications citing this paper.
Showing 1-10 of 50 extracted citations

References

Publications referenced by this paper.
Showing 1-10 of 29 references

A revised medium for rapid growth and bio assays with tobacco tissue cultures

T Murashige, F Skoog
Physiol Plant • 1962
View 2 Excerpts
Highly Influenced

Selection of marker-free transgenic plants using the isopentenyl transferase gene.

Proceedings of the National Academy of Sciences of the United States of America • 1997
View 4 Excerpts

Cre recombinase-mediated site-specific recombination between plant chromosomes.

Proceedings of the National Academy of Sciences of the United States of America • 1994
View 3 Excerpts

Inactivation of repeated genes – DNA - DNA interaction ?

M Matzke, Matzke AJM, OM Scheid
1994
View 1 Excerpt

Inactivation of repeated genes – DNA-DNA interaction? In: Paszkowski J (ed) Homologous recombination and gene silencing in plants

M Matzke, AJM Matzke, OM Scheid
1994
View 1 Excerpt

Similar Papers

Loading similar papers…