Effective mutagenesis of Vibrio fischeri by using hyperactive mini-Tn5 derivatives.

Abstract

We have developed a transposon mutagenesis system for Vibrio fischeri ES114 that utilizes a hyperactive mutant Tn5 transposase (E54K and M56A) and optimized transposon ends. Using a conjugation-based procedure, we obtained independent single-insertion mini-Tn5 mutants at a rate of approximately 10(-6). This simple and inexpensive technique represents a… (More)
DOI: 10.1128/AEM.01330-08

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