Effective gene suppression using small interfering RNA in hard-to-transfect human T cells.

@article{Yin2006EffectiveGS,
  title={Effective gene suppression using small interfering RNA in hard-to-transfect human T cells.},
  author={Jiyi Yin and Zhengyu Ma and Nithianandan Selliah and Debra K. Shivers and Randy Q. Cron and Terri Helman Finkel},
  journal={Journal of immunological methods},
  year={2006},
  volume={312 1-2},
  pages={
          1-11
        }
}
RNA interference (RNAi) is an evolutionarily conserved cellular defense mechanism that protects cells from hostile genes and regulates the function of normal genes during growth and development. In this study, we established proof of principle of small interfering RNA (siRNA) silencing in hard-to-transfect human T cell lines and primary human CD4 T cells. We used public and in-house programs to design four siRNAs each for GFP, for our novel cellular gene HALP, and for their corresponding… CONTINUE READING

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