Effective expression and purification of recombinant onconase, an antitumor protein.

@article{Notomista1999EffectiveEA,
  title={Effective expression and purification of recombinant onconase, an antitumor protein.},
  author={Eugenio Notomista and Valeria Cafaro and R Fusiello and Aurora Bracale and G D'alessio and Alberto Di Donato},
  journal={FEBS letters},
  year={1999},
  volume={463 3},
  pages={211-5}
}
Several members of the RNase A superfamily are endowed with antitumor activity, showing selective cytotoxicity toward several tumor cell lines. One of these is onconase, the smallest member of the RNase A superfamily, which is at present undergoing phase III clinical trials. We report here the expression of recombinant onconase in Escherichia coli inclusion bodies, the correct processing of the protein, followed by its purification in high yields. The recombinant protein has biological and… CONTINUE READING

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