Effective RNAi-mediated gene silencing without interruption of the endogenous microRNA pathway

@article{John2007EffectiveRG,
  title={Effective RNAi-mediated gene silencing without interruption of the endogenous microRNA pathway},
  author={Matthias John and Rainer Constien and Akin Akinc and Michael Goldberg and Young Ah Moon and Martina Spranger and Philipp Hadwiger and J{\"u}rgen Soutschek and Hans-Peter Vornlocher and Muthiah Manoharan and Markus Stoffel and Robert Langer and Daniel G Anderson and Jay D. Horton and Victor Koteliansky and David A. Bumcrot},
  journal={Nature},
  year={2007},
  volume={449},
  pages={745-747}
}
Systemic administration of synthetic small interfering RNAs (siRNAs) effectively silences hepatocyte gene expression in rodents and primates. Whether or not in vivo gene silencing by synthetic siRNA can disrupt the endogenous microRNA (miRNA) pathway remains to be addressed. Here we show that effective target-gene silencing in the mouse and hamster liver can be achieved by systemic administration of synthetic siRNA without any demonstrable effect on miRNA levels or activity. Indeed, siRNA… CONTINUE READING

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