Effective DNA fragmentation technique for simple sequence repeat detection with a microsatellite-enriched library and high-throughput sequencing.

Abstract

Two different techniques for genomic DNA fragmentation before microsatellite-enriched library construction-restriction enzyme (NlaIII and MseI) digestion and sonication-were compared to examine their effects on simple sequence repeat (SSR) detection using high-throughput sequencing. Tens of thousands of SSR regions from 5 species of the plant family… (More)
DOI: 10.2144/000114536

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Cite this paper

@article{Tanaka2017EffectiveDF, title={Effective DNA fragmentation technique for simple sequence repeat detection with a microsatellite-enriched library and high-throughput sequencing.}, author={Keisuke Tanaka and Rumi Ohtake and Saki Yoshida and Takashi Shinohara}, journal={BioTechniques}, year={2017}, volume={62 4}, pages={180-182} }