[Effect on apoptosis of anterior pituitary induced by cadmium chloride and its relations with p38 MAPK & ERK1/2 passway].

Abstract

OBJECTIVE To study the effects of cadmium chloride on anterior pituitary and the relation between p38 MAPK & ERK1/2 pathway and apoptosis induced by cadmium chloride. METHODS In vivo studies, SD male rats were administered with CdCl2 at the doses of 0, 1.0, 2.0, 4.0 mg/kg bw by gavage for 6 weeks. In vitro studies, rats' anterior pituitary cells were primarily cultured and then treated with CdCl2 at doses of 0, 1.56, 3.12, 6.25, 12.50, 25.00, 50.00, 100.00 micromol/L for 6 hours. Inhabiting study, cells were incubated with SB203580 (2.65 micromol/L) or U0126 (10 micromol/L) respectively for 2 hours and then treated with CdCl2 at doses of 3.12, 100.00 micromol/L for 6 hours. The indices in above experiments included expression of TUNEL-positive cells, rate of apoptosis by Flow cytometry. RESULTS The results showed that the apoptosis induced by CdCl2 inclined to enhance with dose dependent manner, Further more, U0126 and SB203580, the specific inhibitor of ERK1/2 and p38 MAPK respectively, could decrease the expression of TUNEL-positive cells significantly and lower apoptotic rate of primary cultured anterior pituitary cells. CONCLUSION It was suggested that cadmium induce apoptosis of anterior pituitary both in vivo and in vitro in dose-dependent manner, ERK1/2 and p38 MAPK passways might play a role during above processes.

Statistics

0100200200920102011201220132014201520162017
Citations per Year

89 Citations

Semantic Scholar estimates that this publication has 89 citations based on the available data.

See our FAQ for additional information.

Cite this paper

@article{Yang2005EffectOA, title={[Effect on apoptosis of anterior pituitary induced by cadmium chloride and its relations with p38 MAPK & ERK1/2 passway].}, author={Xing-fen Yang and Wei Zhu and Qing Wei and Zhong-ning Lin}, journal={Wei sheng yan jiu = Journal of hygiene research}, year={2005}, volume={34 6}, pages={681-4} }