Effect of zinc binding and precipitation on structures of recombinant human growth hormone and nerve growth factor.

@article{Yang2000EffectOZ,
  title={Effect of zinc binding and precipitation on structures of recombinant human growth hormone and nerve growth factor.},
  author={T H Yang and Jeffrey L. Cleland and X Lam and Jeffrey D. Meyer and Latoya S Jones and Theodore W. Randolph and Mark Cornell Manning and John F. Carpenter},
  journal={Journal of pharmaceutical sciences},
  year={2000},
  volume={89 11},
  pages={1480-5}
}
Metal-induced precipitation of protein therapeutics is being used and further developed as a processing step in protein formulation and may have utility in protein purification and bulk storage. In such processes, it is imperative that native protein structure is maintained and the metal complexation is reversible. In the current study, we investigated the effects of zinc-induced precipitation on recombinant human growth hormone (rhGH) and recombinant human nerve growth factor (rhNGF). On the… CONTINUE READING