Effect of zeta potential of cationic liposomes containing cationic cholesterol derivatives on gene transfection

  title={Effect of zeta potential of cationic liposomes containing cationic cholesterol derivatives on gene transfection},
  author={Keiji Takeuchi and Masao Ishihara and Chiyo Kawaura and M Noji and Tadahide Furuno and Mamoru Nakanishi},
  journal={FEBS Letters},
Cationic liposomes are known to be useful tools for gene transfection. However, the relation between transfection efficiency and physicochemical properties of liposomes has not been well understood. Here, we synthesized eight cationic derivatives of cholesterol which contain a tertiary amino head group with a different spacer arm. Transfection of plasmid pSV2CAT DNA into cells was done by cationic liposomes made of a mixture of dioleoylphosphatidylethanolamine (DOPE) and each cationic… 
Confocal and probe microscopy to study gene transfection mediated by cationic liposomes with a cationic cholesterol derivative.
A novel cationic cholesterol derivative with a hydroxyethyl amino head group (I) has been synthesized and used for liposome-mediated gene transfection and it was found that microtubules were involved in the intracellular dynamics of genetransfection.
Cationic Liposomes for Gene Transfection
In vitro transfection efficiency by liposomes/plasmid pCMVβ complexes was found to depend on the kind of lipid associated in theliposomes and the lipOSomes/DNA mixing ratio, and the importance of associating DOPE in cationic liposome was confirmed.
Efficient Delivery of Plasmid DNA Using Cholesterol-Based Cationic Lipids Containing Polyamines and Ether Linkages
  • B. Kim, Y. Seu, +6 authors K. Doh
  • Biology, Medicine
    International journal of molecular sciences
  • 2014
Under optimal conditions, each liposome formulation showed higher transfection efficiency in AGS and Huh-7 cells than commercially available cationic liposomes, particularly in the presence of serum.
Atomic force microscopy for studying gene transfection mediated by cationic liposomes with a cationic cholesterol derivative
From the results it was found that the vesicles with moderate diameters were moste effective for gene transfection of plasmid pSV2CAT DNA into the target cell.
Comparative study of transfection efficiency of cationic cholesterols mediated by liposomes-based gene delivery.
It is found that the stability of the liposome-DNA complex in the presence of endosome deeply related to the transfection efficiency, and the introduction of a hydrophilic group to the amino terminal of the cholesterol derivative decreased stability and facilitated the release of DNA from the endosomes, resulting in higher transfections efficiency.
Preparation, characterization, transfection efficiency, and cytotoxicity of liposomes containing oligoamine-modified cholesterols as nanocarriers to Neuro2A cells.
The designed systems are able to transfer the DNA into Neuro2A cells with low or no toxicity, and the ideal parameters for preparing large unilamellar vesicles as nanocarriers containing oligoamine-modified cholesterols are described.
Membrane fusion plays an important role in gene transfection mediated by cationic liposomes
The results suggest that at least two steps are effective for gene transfection mediated by the cationic liposomes with cATIONic cholesterol derivatives, including the endocytosis of the liposome/DNA complex into the target cells and the removal of antisense oligonucleotides (plasmid DNAs) from the complex in the endosomes.
Chapter 5: Cationic Liposomes as Transmembrane Carriers of Nucleic Acids
Abstract Cationic liposomes constitute a new class of bilayer, which are able to form complexes with nucleic acids (DNA or RNA), help to cross the cellular membranes, and then deliver them into
Cationic cholesterol with a hydroxyethylamino head group promotes significantly liposome‐mediated gene transfection
A novel cationic cholesterol derivative with a hydroxyethylamino head group, cholesteryl‐3β‐carboxyamidoethylene‐N‐hydroxyethylamine (II), has been synthesized and used for liposome‐mediated gene transfection and facilitated greatly pSV2CAT gene transference into mouse NIH3T3 and L929 cells in the absence of serum.
Cationic lipids: molecular structure/ transfection activity relationships and interactions with biomembranes.
It is demonstrated that optimum transfection is displayed by lipids with chain length of approximately 14 carbon atoms and that the transfections efficiency strongly increases with increase of chain unsaturation, specifically upon replacement of saturated with monounsaturated chains.


The role of dioleoyl phosphatidylethanolamine in cationic liposome mediated gene transfer.
An endocytosis model of DNA delivery by cationic liposomes is proposed in which the principal function of the chase liposome is to destabilize the endosome membrane and allow the release of DNA into the cytosol.
Effect of cationic cholesterol derivatives on gene transfer and protein kinase C activity.
Four different cationic derivatives of cholesterol were synthesized which contain either a tertiary or a quaternary amino head group, with and without a succinyl spacer-arm, and several adherent cell lines could be efficiently transfected with this liposome reagent without any apparent cytotoxicity.
Use of a quaternary ammonium detergent in liposome mediated DNA transfection of mouse L-cells.
Although the overall transfection activity of the liposome containing the quaternary ammonium detergent is somewhat lower than that of the Lipofectin, it may serve as an inexpensive and convenient alternative.
A novel cationic liposome reagent for efficient transfection of mammalian cells.
  • X. Gao, L. Huang
  • Chemistry, Medicine
    Biochemical and biophysical research communications
  • 1991
A novel cationic derivative of cholesterol, 3 beta [N-(N',N'-dimethylaminoethane)-carbamoyl] cholesterol (DC-Chol), has been synthesized and used to prepare sonicated liposomes with dioleoylphosphatidylethanolamine and facilitates efficient DNA mediated transfection in A431 human epidermoid carcinoma cells.
Cationic liposomes improve stability and intracellular delivery of antisense oligonucleotides into CaSki cells.
Improved stability in the culture medium indicates that the cationic liposomes per se protect the ODNs from enzymatic degradation, and improved cellular delivery was largely due to the stabilization of ODNs by cationo-liposomes.
Lipofection: a highly efficient, lipid-mediated DNA-transfection procedure.
Depending upon the cell line, lipofection is from 5- to greater than 100-fold more effective than either the calcium phosphate or the DEAE-dextran transfection technique.
Fusion of cationic liposomes with mammalian cells occurs after endocytosis.
It is demonstrated that binding to the cell surface is insufficient for cationic liposome-cell fusion and that uptake into the endocytic pathway is required for fusion to occur.
Cationic liposome-mediated RNA transfection.
We have developed an efficient and reproducible method for RNA transfection, using a synthetic cationic lipid, N-[1-(2,3-dioleyloxy)propyl]-N,N,N-trimethylammonium chloride (DOTMA), incorporated into
Drug-induced zeta potential changes in liposomes studied by laser Doppler spectroscopy.
The technique of laser Doppler spectroscopy is used to measure the electrophoretic mobility of liposomes under the influence of one beta-blocking agent and three local anesthetics, indicating binding and incorporation of positively charged drug molecules.
Cationic lipids enhance cellular uptake and activity of phosphorothioate antisense oligonucleotides.
It is demonstrated that cationic lipids increase antisense activity by increasing the amount of oligonucleotide associated with cells and altering intracellular distribution of the oligon nucleotide.