Effect of zeta potential of cationic liposomes containing cationic cholesterol derivatives on gene transfection

@article{Takeuchi1996EffectOZ,
  title={Effect of zeta potential of cationic liposomes containing cationic cholesterol derivatives on gene transfection},
  author={Keiji Takeuchi and Masao Ishihara and Chiyo Kawaura and M Noji and Tadahide Furuno and Mamoru Nakanishi},
  journal={FEBS Letters},
  year={1996},
  volume={397}
}
Cationic liposomes are known to be useful tools for gene transfection. However, the relation between transfection efficiency and physicochemical properties of liposomes has not been well understood. Here, we synthesized eight cationic derivatives of cholesterol which contain a tertiary amino head group with a different spacer arm. Transfection of plasmid pSV2CAT DNA into cells was done by cationic liposomes made of a mixture of dioleoylphosphatidylethanolamine (DOPE) and each cationic… 
Confocal and probe microscopy to study gene transfection mediated by cationic liposomes with a cationic cholesterol derivative.
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A novel cationic cholesterol derivative with a hydroxyethyl amino head group (I) has been synthesized and used for liposome-mediated gene transfection and it was found that microtubules were involved in the intracellular dynamics of genetransfection.
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In vitro transfection efficiency by liposomes/plasmid pCMVβ complexes was found to depend on the kind of lipid associated in theliposomes and the lipOSomes/DNA mixing ratio, and the importance of associating DOPE in cationic liposome was confirmed.
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From the results it was found that the vesicles with moderate diameters were moste effective for gene transfection of plasmid pSV2CAT DNA into the target cell.
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Cationic cholesterol with a hydroxyethylamino head group promotes significantly liposome‐mediated gene transfection
TLDR
A novel cationic cholesterol derivative with a hydroxyethylamino head group, cholesteryl‐3β‐carboxyamidoethylene‐N‐hydroxyethylamine (II), has been synthesized and used for liposome‐mediated gene transfection and facilitated greatly pSV2CAT gene transference into mouse NIH3T3 and L929 cells in the absence of serum.
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It is demonstrated that optimum transfection is displayed by lipids with chain length of approximately 14 carbon atoms and that the transfections efficiency strongly increases with increase of chain unsaturation, specifically upon replacement of saturated with monounsaturated chains.
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