Effect of vitamin C and vitamin E analog on aged fibroblasts.

  title={Effect of vitamin C and vitamin E analog on aged fibroblasts.},
  author={Mireia Farriol and M. Lourdes Mourelle and Sherwyn Schwartz},
  journal={Revista espanola de fisiologia},
  volume={50 4},
Human dermal fibroblasts were cultured and aged in vitro. Survival of young and aged fibroblasts was determined in the presence and absence of different concentrations of two vitamins. Vit C at doses of 5, 12.5, 25 and 50 mumol/L and water-soluble Vit E (Trolox) at 1, 5, 10 and 50 mg/L, were added 30 minutes before oxidative stress, consisting of exposure to 5 mM hydrogen peroxide for 30 minutes. A non-radioactive cell proliferation cytotoxicity assay (MTT) was used to determine the protective… CONTINUE READING
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