Effect of various freezing parameters on the immediate post-thaw membrane integrity of adipose tissue derived adult stem cells.

@article{Thirumala2005EffectOV,
  title={Effect of various freezing parameters on the immediate post-thaw membrane integrity of adipose tissue derived adult stem cells.},
  author={Sreedhar Thirumala and Sanjin Zvonic and Elizabeth Z Floyd and Jeffrey M. Gimble and Ram V. Devireddy},
  journal={Biotechnology progress},
  year={2005},
  volume={21 5},
  pages={1511-24}
}
The effect of four thermal parameters on post-thaw membrane integrity of adipose tissue derived adult stem (ADAS) cells after controlled-rate freezing was investigated with the help of a two-level four-parameter (2(4)) experimental design. The four thermal parameters studied were cooling rate (CR), end temperature (ET), hold time (HT), and thawing rate (TR). Several passages, including Passage-0 (P0), Passage-1 (P1), Passage-2 (P2), Passage-3 (P3), and Passage-4 (P4), obtained from the… CONTINUE READING

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