Effect of various cooling rates (from 30 degrees C to 5 degrees C) and thawing temperatures on the deep-freezing of Bos Taurus and Bos Bubalis semen.

@article{Dhami1992EffectOV,
  title={Effect of various cooling rates (from 30 degrees C to 5 degrees C) and thawing temperatures on the deep-freezing of Bos Taurus and Bos Bubalis semen.},
  author={A J Dhami and Karan Sahni and Govind Mohan},
  journal={Theriogenology},
  year={1992},
  volume={38 3},
  pages={565-74}
}
A total of 36 semen ejaculates, six from each of three Holstein-Friesian bulls and three Murrah buffalo bulls, were frozen in tris citric acid-fructose-egg-yolk-glycerol diluent after 1 hour of equilibration to study the effect of various cooling rates (15, 30, 60 and 120 minutes from 10 degrees to 5 degrees C vs a control sample cooled for 120 minutes from 28 degrees to 5 degrees C) and thawing temperatures (40 degrees C 60 seconds , 60 degrees C 15 seconds and 80 degrees C 5 seconds ) on… CONTINUE READING