Effect of vaccination with a recombinant metalloprotease from Haemaphysalis longicornis

  title={Effect of vaccination with a recombinant metalloprotease from Haemaphysalis longicornis},
  author={Saiki Imamura and Itabajara da Silva Vaz and Satoru Konnai and Shinji Yamada and Chie Nakajima and Misao Onuma and Kazuhiko Ohashi},
  journal={Experimental and Applied Acarology},
We report the cloning, expression and characterization of an Haemaphysalis longicornis metalloprotease (named HLMP1). The gene encodes a predicted 550 aminoacid protein with similarity to metalloproteases of the reprolysin family. The protein sequence contains a signal sequence, the zinc-binding motif (HEXXHXXGXXH) common to metalloproteases and a cysteine-rich region. Reverse transcription-PCR expression analysis indicates the presence of mRNA in the salivary gland of larva, nymph and adult… CONTINUE READING

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