Effect of transformation ofAzotobacter vinelandii with the low copy number plasmid pRK290

  title={Effect of transformation ofAzotobacter vinelandii with the low copy number plasmid pRK290},
  author={Dr. Bernard R. Glick and Barbara J. ButlerB.J. Butler and Colin I. Mayfield and Justyna Pasternak},
  journal={Current Microbiology},
We previously observed that whenAzotobacter vinelandii was transformed by different broad-host-range plasmids, normal cellular functions such as growth and siderophore production are impaired. In the present work, whenA. vinelandii was transformed with the low copy number plasmid pRK290, the extent of this metabolic impairment was lessened, as evidenced by increased siderophore production and moderate levels of growth on medium that lacks added iron. It is concluded that the severity of the… CONTINUE READING

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