Effect of transfection and passage number of ear fibroblasts on in vitro development of bovine transgenic nuclear transfer embryos.

@article{Bhuiyan2004EffectOT,
  title={Effect of transfection and passage number of ear fibroblasts on in vitro development of bovine transgenic nuclear transfer embryos.},
  author={M M Uddin Bhuiyan and Jongki Cho and Goo Jang and Eulsoon Park and Sungkeun Kang and Byeongchun Lee and Woosuk Hwang},
  journal={The Journal of veterinary medical science},
  year={2004},
  volume={66 3},
  pages={257-61}
}
The objective of this study was to determine if the transfection of human prourokinase (ProU) gene and passage number of transfected ear fibroblasts affected in vitro development of bovine transgenic nuclear transfer (NT) embryos. An expression plasmid for human ProU was constructed by inserting a bovine beta-casein promoter, a green fluorescent protein (GFP) marker and human ProU gene into a pcDNA3 plasmid and transfected into bovine ear fibroblasts using a lipid mediated method. Abattoir… CONTINUE READING