Effect of tissue fixation on recovery of DNA adducts in the 32P-postlabelling assay.

The suitability of fixed tissue as a source of DNA for 32P-postlabelling studies on DNA adduct formation has been investigated. Tissues (spleen, liver, lung, colon and kidney) from rats treated i.p. with benzo[a]pyrene (BaP) (100 mg/kg) or 2-acetylaminofluorene (AAF) (40 mg/kg) were removed and placed in formalin fixative for 1, 7, 28 or 92 days. DNA was… CONTINUE READING