Effect of the pulse length of ultrasound on cell membrane damage in vitro.

Abstract

Suspended cells of a human lymphoblastic cell line were exposed to pulsed ultrasound of 775 kHz. The pulse lengths were varied between 16 and 1000 microseconds. The mark/space ratio was always kept at 1:1. Two ultrasound intensity levels were used: 3.6 and 6.4 W/cm2 spatial peak and temporal peak. After an exposure time of 5 min, cell membrane damage was measured cytometrically by a dye exclusion test. No membrane damage was observable at 16 microseconds, whereas, at pulse lengths of 1000 microseconds, about one-third of the cells were damaged.

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@article{Kober1989EffectOT, title={Effect of the pulse length of ultrasound on cell membrane damage in vitro.}, author={L O Kober and J. Ellwart and Hans Brettel}, journal={The Journal of the Acoustical Society of America}, year={1989}, volume={86 1}, pages={6-7} }