Effect of the protein corona on nanoparticles for modulating cytotoxicity and immunotoxicity

  title={Effect of the protein corona on nanoparticles for modulating cytotoxicity and immunotoxicity},
  author={Yeon Kyung Lee and Eun-Ju Choi and Thomas Jay Webster and Sang-Hyun Kim and Dongwoo Khang},
  journal={International Journal of Nanomedicine},
  pages={97 - 113}
Although the cytotoxicity of nanoparticles (NPs) is greatly influenced by their interactions with blood proteins, toxic effects resulting from blood interactions are often ignored in the development and use of nanostructured biomaterials for in vivo applications. Protein coronas created during the initial reaction with NPs can determine the subsequent immunological cascade, and protein coronas formed on NPs can either stimulate or mitigate the immune response. Along these lines, the… 
The impact of nanoparticle protein corona on cytotoxicity, immunotoxicity and target drug delivery.
A survey of recent findings on the NP-PC interactions is provided and how the PC can be used to modulate both cytotoxicity and the immune response as well as to improve the efficacy of targeted delivery of nanocarriers is discussed.
Plasma protein adsorption and biological identity of systemically administered nanoparticles.
The fundamentals of NP-plasma protein interaction, the consequences of the interactions, and insights into the correlations of protein corona with biodistribution and cellular delivery are reviewed.
Protein–Nanoparticle Interaction: Corona Formation and Conformational Changes in Proteins on Nanoparticles
Understanding of the conformational changes and unfolding process of proteins is very important to accelerate the biomedical applications of NPs.
Nanoparticle-Protein Interaction: The Significance and Role of Protein Corona.
This chapter describes the formation of protein corona on nanoparticles and the differences arising in its composition due to variations in nanoparticle properties.
A health concern regarding the protein corona, aggregation and disaggregation
Protein corona: a new approach for nanomedicine design
This review focuses on the formation of protein corona and its potential applications in pharmaceutical sciences such as prediction modeling based on NP-adsorbed proteins, usage of active proteins for modifying NP to achieve toxicity reduction, circulation time enhancement, and targeting effect.
Interactions of organic nanoparticles with proteins in physiological conditions.
This review will focus on studies of protein corona formation onto the soft, organic-based NPs, upon incubation in biological media such as human plasma or serum and their physicochemical characteristics.
Understanding the Lipid and Protein Corona Formation on Different Sized Polymeric Nanoparticles
It is observed that cholesterol and triglycerides effectively bind to NP emphasizing that proteins are not the only biomolecules with high-affinity binding to nanomaterial surfaces and that further knowledge on NP interactions with mouse serum is necessary regarding the common use of this model to predict the in vivo efficiency of NP.


Interactions of nanoparticles with plasma proteins: implication on clearance and toxicity of drug delivery systems
The immune recognition of nanoparticles can seriously affect the drug delivery efficacy and toxicity, and there is at present not enough knowledge on the mechanisms that dictate the nanoparticle immune recognition and stability in the biological milieu.
Impact of protein modification on the protein corona on nanoparticles and nanoparticle-cell interactions.
Recent studies have firmly established that cellular uptake of nanoparticles is strongly affected by the presence and the physicochemical properties of a protein adsorption layer around these
Biosafety and bioapplication of nanomaterials by designing protein-nanoparticle interactions.
The protein-nanoparticle (NP) interface is a current frontier of multiple disciplines, full of challenges and opportunities. The unique behaviors of nanomaterials (NMs) bring many exciting
Polymer-coated nanoparticles interacting with proteins and cells: focusing on the sign of the net charge.
This study shows that the number of adsorbed human serum albumin molecules per NP was not influenced by their surface charge, and cytot toxicity assays revealed a higher cytotoxicity for positively charged NPs, associated with their enhanced uptake.
Biomolecular coronas provide the biological identity of nanosized materials.
The basic concept of the nanoparticle corona is reviewed and its structure and composition is highlighted, and how the properties of the corona may be linked to its biological impacts are highlighted.
Hardening of the nanoparticle-protein corona in metal (Au, Ag) and oxide (Fe3O4, CoO, and CeO2) nanoparticles.
Results show that production of reactive oxygen species is decreased if the nanoparticles are preincubated for 48 h with serum, and different temporal patterns of the protein corona formation are observed that can be considered a fingerprint for nanoparticle identification.
Physical-chemical aspects of protein corona: relevance to in vitro and in vivo biological impacts of nanoparticles.
The hard corona can evolve quite significantly as one passes from protein concentrations appropriate to in vitro cell studies to those present in in vivo studies, which has deep implications for in vitro-in vivo extrapolations and will require some consideration in the future.
Hard corona composition and cellular toxicities of the graphene sheets.
Nanoparticle size and surface properties determine the protein corona with possible implications for biological impacts
The long-lived (“hard”) protein corona formed from human plasma is studied for a range of nanoparticles that differ in surface properties and size and both size and surface properties were found to play a very significant role.
Understanding the nanoparticle–protein corona using methods to quantify exchange rates and affinities of proteins for nanoparticles
The rates of protein association and dissociation are determined using surface plasmon resonance technology with nanoparticles that are thiol-linked to gold, and through size exclusion chromatography of protein–nanoparticle mixtures, and this method is developed into a systematic methodology to isolate nanoparticle-associated proteins.