Effect of the mobile phase composition on the isotherm parameters and the high concentration band profiles in reversed-phase liquid chromatography.


Single-component adsorption isotherm data were acquired by frontal analysis for phenol on a C18-Kromasil packed column, under reversed-phase liquid chromatography conditions, using various methanol-water solutions (30-60%, v/v, methanol). The isotherm model accounting best for these data was the biLangmuir model. With increasing methanol content, the two… (More)