Effect of the increased stability of the penicillin amidase mRNA on the protein expression levels.

@article{Viegas2005EffectOT,
  title={Effect of the increased stability of the penicillin amidase mRNA on the protein expression levels.},
  author={Sandra C Viegas and Dorothea F Schmidt and Volker Kasche and Cecilia Maria Arraiano and Zoya Ignatova},
  journal={FEBS letters},
  year={2005},
  volume={579 22},
  pages={
          5069-73
        }
}
Several factors at transcriptional, post-transcriptional or post-translational level determine the fate of a target protein and can severely restrict its yield. Here, we focus on the post-transcriptional regulation of the biosynthesis of the periplasmic protein, penicillin amidase (PA). The PA mRNA stability was determined under depleted RNase conditions in strains carrying single or multiple RNase deletions. Single deletion of the endonuclease RNase E yielded, as the highest, a fourfold… CONTINUE READING
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