Effect of the immobilisation of DNA aptamers on the detection of thrombin by means of surface plasmon resonance.

@article{Ostatn2008EffectOT,
  title={Effect of the immobilisation of DNA aptamers on the detection of thrombin by means of surface plasmon resonance.},
  author={Veronika Ostatn{\'a} and Hana Vaisocherov{\'a} and Jiř{\'i} Homola and Tibor Hianik},
  journal={Analytical and bioanalytical chemistry},
  year={2008},
  volume={391 5},
  pages={1861-9}
}
We report a multichannel surface plasmon resonance (SPR) sensor for detection of thrombin via DNA aptamers immobilized on the SPR sensor surface. A detailed investigation of the effect of the immobilisation method on the interaction between thrombin and DNA aptamers is presented. Three basic approaches to the immobilisation of aptamers on the surface of the SPR sensor are examined: (i) immobilisation based on chemisorption of aptamers modified with SH groups, (ii) immobilisation of biotin… CONTINUE READING

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