Effect of the gastrointestinal environment on pH homeostasis of Lactobacillus plantarum and Lactobacillus brevis cells as measured by real-time fluorescence ratio-imaging microscopy.

@article{Ramos2014EffectOT,
  title={Effect of the gastrointestinal environment on pH homeostasis of Lactobacillus plantarum and Lactobacillus brevis cells as measured by real-time fluorescence ratio-imaging microscopy.},
  author={C{\'i}ntia Lacerda Ramos and Line Thorsen and Mia Ryssel and Dennis Sandris Nielsen and Henrik Siegumfeldt and Rosane Freitas Schwan and Lene Jespersen},
  journal={Research in microbiology},
  year={2014},
  volume={165 3},
  pages={215-25}
}
In the present work, an in vitro model of the gastrointestinal tract (GIT) was developed to obtain real-time observations of the pH homeostasis of single cells of probiotic Lactobacillus spp. strains as a measure of their physiological state. Changes in the intracellular pH (pHi) were determined using fluorescence ratio imaging microscopy (FRIM) for potential probiotic strains of Lactobacillus plantarum UFLA CH3 and Lactobacillus brevis UFLA FFC199. Heterogeneous populations were observed, with… CONTINUE READING