Effect of the exposure to methyl-β-cyclodextrin prior to chilling or vitrification on the viability of bovine immature oocytes.

@article{Spricigo2012EffectOT,
  title={Effect of the exposure to methyl-β-cyclodextrin prior to chilling or vitrification on the viability of bovine immature oocytes.},
  author={Jos{\'e} Felipe Warmling Spricigo and Karollyne S Morais and B S Yang and Margot Alves Nunes Dode},
  journal={Cryobiology},
  year={2012},
  volume={65 3},
  pages={319-25}
}
The present study aimed to evaluate the effect of methyl-β-cyclodextrin (MβCD) as a cholesterol loader to change oocyte plasma membrane and increase its tolerance toward cryopreservation. The first and second experiments were conducted to investigate if MβCD could improve nuclear and cytoplasmic maturation after oocyte exposure to cold stress for 10 or 30 min, respectively. No differences (P>0.05) in either experiment in the metaphase II (MII) rate of oocytes exposed to MβCD and cold stress… CONTINUE READING
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