Effect of the content of unmethylated CpG dinucleotides in plasmid DNA on the sustainability of transgene expression.

@article{Mitsui2009EffectOT,
  title={Effect of the content of unmethylated CpG dinucleotides in plasmid DNA on the sustainability of transgene expression.},
  author={Masaru Mitsui and Makiya Nishikawa and Lei Zang and Mitsuru Ando and Kayoko Hattori and Yuki Takahashi and Yoshihiko Watanabe and Yoshinobu Takakura},
  journal={The journal of gene medicine},
  year={2009},
  volume={11 5},
  pages={435-43}
}
BACKGROUND Nonviral gene transfer generally suffers from short-term expression of transgenes. We have previously demonstrated that plasmids with reduced CpG content exhibited a more prolonged expression of murine interferon (IFN)-beta or IFN-gamma, which was effective in inhibiting metastatic tumor growth. A further extension of the duration of transgene expression could be achieved by controlling the number and location of CpG motifs in plasmid DNA. METHODS Luciferase-expressing plasmids… CONTINUE READING

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