Effect of the β‐glucuronidase inhibitor saccharolactone on glucuronidation by human tissue microsomes and recombinant UDP‐glucuronosyltransferases

@article{Oleson2008EffectOT,
  title={Effect of the $\beta$‐glucuronidase inhibitor saccharolactone on glucuronidation by human tissue microsomes and recombinant UDP‐glucuronosyltransferases},
  author={Lauren E. Oleson and Michael H. Court},
  journal={Journal of Pharmacy and Pharmacology},
  year={2008},
  volume={60}
}
  • L. Oleson, M. Court
  • Published 1 September 2008
  • Biology
  • Journal of Pharmacy and Pharmacology
Glucuronidation studies using microsomes and recombinant uridine diphosphoglucuronosyltransferases (UGTs) can be complicated by the presence of endogenous β‐glucuronidases, leading to underestimation of glucuronide formation rates. Saccharolactone is the most frequently used β‐glucuronidase inhibitor, although it is not clear whether this reagent should be added routinely to glucuronidation incubations. Here we have determined the effect of saccharolactone on eight different UGT probe… 
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