[Effect of temperature and concentrations of initial components on synthesis of internucleotide bond catalyzed by pancreatic ribonuclease].

Abstract

The synthesis of cytidylyu-(3,-5,)-cytidine (CpC) catalyzed by pancreatic ribonuclease at 23 degrees, 0 degrees, and -15 degrees C in Tris-HCl-buffer was compared with that in aqueous propan-2-0. The data obtained show that the increase in the yield of oligonucleotides in aqueous buffer at -15 degrees, observed earlier is rather a result of the concentration change in the reaction mixture caused by the freezing of water than by a temperature fall from 0 to -15 degrees. A 4-fold increase in the initial concentrations of the substrates and ribonuclease with respect to the concentrations used earlier leads to the yield of CC in a homogeneous solution at 0 degrees close to is yield found in the frozen mixture at -15 degrees.

Cite this paper

@article{Kavunenko1975EffectOT, title={[Effect of temperature and concentrations of initial components on synthesis of internucleotide bond catalyzed by pancreatic ribonuclease].}, author={A P Kavunenko and T N Kalacheva and N. S. Tikhomirova-Sidorova and L M Iakunitskaia}, journal={Biokhimii︠a︡}, year={1975}, volume={40 4}, pages={755-61} }