Effect of supplemetation of Zebularine and Scriptaid on efficiency of in vitro developmental competence of ovine somatic cell nuclear transferred embryos

  title={Effect of supplemetation of Zebularine and Scriptaid on efficiency of in vitro developmental competence of ovine somatic cell nuclear transferred embryos},
  author={Xiaohua Fang and W. Xia and H. Cao and Y. Guo and Hanyang Wang and Xiaosheng Zhang and P. Wan and Chuang Liu and Qiaoli Wei and Shuchun Sun and Shujun Tian and Junjie Li and Zhigang Wang},
  journal={Animal Biotechnology},
  pages={155 - 163}
Abstract Somatic cell nuclear transfer (SCNT) technology has been applied in the construction of disease model, production of transgenic animals, therapeutic cloning, and other fields. However, the cloning efficiency remains limited. In our study, to improve SCNT efficiency, brilliant cresyl blue (BCB) staining were chosen to select recipient oocytes. In addition, DNA methyltransferase inhibitor Zebularine (5 nmol/L) and histone deacetylase inhibitor Scriptaid (0.2 μmol/L) were jointly used to… Expand
4 Citations
Enhancement of epigenetic reprogramming status of porcine cloned embryos with zebularine, a DNA methyltransferase inhibitor
It is found that Zb could modify DNA methylation levels in the early stages of porcine SCNT embryos and promote their developmental competence. Expand
In vitro production of canine blastocysts.
This is the first in vitro canine preimplantation embryo culture system that can successfully produce canine blastocysts, and it is found that blastocyst formation in PA embryos cultured in QAU-4 medium was quite high, though this was not so in the case of SCNT embryos. Expand
Manipulating the Epigenome in Nuclear Transfer Cloning: Where, When and How
This review describes in detail where, when, and how manipulations of the epigenome of donor cells and reconstructed SCNT embryos can be performed to optimize the process of molecular reprogramming and the outcome of nuclear transfer cloning. Expand
In vitro embryos production of oocytes selected based on follicular size and by brilliant cresyl blue staining
We evaluated the competence of bovine oocytes via morphological selection associated with follicular diameter and by brilliant cresyl blue (BCB) staining, in order to improve oocyte selection and theExpand


The histone deacetylase inhibitor Scriptaid improves in vitro developmental competence of ovine somatic cell nuclear transferred embryos.
It is concluded that Scriptaid was more effective than TSA to enhance in vitro developmental competence in ovine SCNT embryos; furthermore, Scriptaid improved epigenetic status. Expand
Scriptaid improves in vitro development and nuclear reprogramming of somatic cell nuclear transfer bovine embryos.
Results indicated that Scriptaid treatment improved the in vitro developmental capacity and the nuclear reprogramming of bovine SCNT embryos. Expand
Zebularine and scriptaid significantly improve epigenetic reprogramming of yak fibroblasts and cloning efficiency.
Functional evidence is provided that treatment with Zebularine and Scriptaid modifies the epigenetic status of yak fibroblasts, subsequently enhancing in vitro developmental potential and the quality of yak cloned embryos. Expand
Effects of the histone deacetylase inhibitor ‘Scriptaid’ on the developmental competence of mouse embryos generated through round spermatid injection
In vitro experiments revealed that treating ROSI mouse zygotes with 250 nM Scriptaid for 10 h significantly improved the blastocyst formation rate and further increased the birth rates of ROSI-derived embryos from 21% to 40% in vivo. Expand
Abnormal levels of transcript abundance of developmentally important genes in various stages of preimplantation bovine somatic cell nuclear transfer embryos.
The results indicate that experiments evaluating gene expression differences between control and SCNT blastocysts may underestimate the degree of difference between clones and controls, and further offer insights into the dynamics of transcript regulation following SCNT. Expand
Methylation characteristics and developmental potential of Guangxi Bama minipig (Sus scrofa domestica) cloned embryos from donor cells treated with trichostatin A and 5-aza-2′-deoxycytidine
Lower concentrations of 5-aza-dC may produce a potent demethylating activity, and lead to the significantly enhanced blastocyst development percentage of Bama minipig SCNT embryos. Expand
Effect of DNA and Histone Methyl Transferase Inhibitors on Outcomes of Buffalo-Bovine Interspecies Somatic Cell Nuclear Transfer.
Investigating whether treating RO with zebularine and BIX-01294 for 16 hours after activation could improve developmental competence and quality of buffalo-bovine interspecies SCNT (iSCNT) embryos found it did not enhance developmental competence of iSCNT embryos, but may have some beneficial effects on epigenetic makeup andquality of derived blastocysts. Expand
Oocytes Selected Using BCB Staining Enhance Nuclear Reprogramming and the In Vivo Development of SCNT Embryos in Cattle
It is found that BCB+ oocytes have a higher nuclear reprogramming capacity, and thatBCB staining can be used to select developmentally competent oocytes for nuclear transfer. Expand
Treating Cloned Embryos, But Not Donor Cells, with 5-aza-2’-deoxycytidine Enhances the Developmental Competence of Porcine Cloned Embryos
TreatingCloned embryos, but not donor cells, with 5-aza-dC enhanced the developmental competence of porcine cloned embryos by promotion of pseudo-pronucleus formation and improvement of pluripotent gene expression. Expand
Scriptaid corrects gene expression of a few aberrantly reprogrammed transcripts in nuclear transfer pig blastocyst stage embryos.
This study identified differentially represented transcripts in pig in vivo derived, in vitro fertilized, or nuclear transfer derived blastocyst stage embryos and the donor cell line by microarray analysis and determined if treatment of reconstructed embryos with the histone deacetylase inhibitor, Scriptaid (NTS), for 14 h postactivation would correct gene expression in a subset of the identified aberrantly reprogrammed transcripts. Expand