Effect of supplemetation of Zebularine and Scriptaid on efficiency of in vitro developmental competence of ovine somatic cell nuclear transferred embryos

@article{Fang2019EffectOS,
  title={Effect of supplemetation of Zebularine and Scriptaid on efficiency of in vitro developmental competence of ovine somatic cell nuclear transferred embryos},
  author={Xiaohua Fang and W. Xia and H. Cao and Y. Guo and Hanyang Wang and Xiaosheng Zhang and P. Wan and Chuang Liu and Qiaoli Wei and Shuchun Sun and Shujun Tian and Junjie Li and Zhigang Wang},
  journal={Animal Biotechnology},
  year={2019},
  volume={31},
  pages={155 - 163}
}
Abstract Somatic cell nuclear transfer (SCNT) technology has been applied in the construction of disease model, production of transgenic animals, therapeutic cloning, and other fields. However, the cloning efficiency remains limited. In our study, to improve SCNT efficiency, brilliant cresyl blue (BCB) staining were chosen to select recipient oocytes. In addition, DNA methyltransferase inhibitor Zebularine (5 nmol/L) and histone deacetylase inhibitor Scriptaid (0.2 μmol/L) were jointly used to… Expand
4 Citations
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This review describes in detail where, when, and how manipulations of the epigenome of donor cells and reconstructed SCNT embryos can be performed to optimize the process of molecular reprogramming and the outcome of nuclear transfer cloning. Expand
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