Effect of substitution of a permeable weak acid for the permissive role of glucose in amino acid-induced electrical activity in B-cells.

@article{Pace1986EffectOS,
  title={Effect of substitution of a permeable weak acid for the permissive role of glucose in amino acid-induced electrical activity in B-cells.},
  author={Caroline S. Pace and Kelly T. Goldsmith},
  journal={Endocrinology},
  year={1986},
  volume={119 6},
  pages={
          2433-8
        }
}
The amino acids L-leucine, L-isoleucine, and L-arginine require a subthreshold concentration of glucose to elicit insulin release and electrical activity from B-cells. There is evidence suggesting that protons couple the metabolism of glucose to the functional response of B-cells. In view of this, a permeable weak acid, sulfamerazine, was used to determine if the generation of intracellular protons could account for the permissive action of glucose. Addition of 10 mM sulfamerazine elicited… 
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ACETAZOLAMIDE SLOWS DOWN THE GLUCOSE-INDUCED MEMBRANE DEPOLARIZATION IN PANCREATIC B-CELLS: THE H+-SENSITIVE K+-PERMEABILITY IS RESPONSIBLE FOR THE DEPOLARIZATION

Findings support the view that a decrease in the H+-sensitive K+-permeability is responsible for the physiological depolarization observed when raising the glucose concentration in the medium.

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Understanding the subjacent mechanisms of each nutrient on β-cells can help to unravel the effects of mixed variables and complexity in the context of β-cell pathology.

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