Effect of storage on the measurement of apolipoproteins A-I and A-II by radial immunodiffusion.

@article{Albers1980EffectOS,
  title={Effect of storage on the measurement of apolipoproteins A-I and A-II by radial immunodiffusion.},
  author={John J. Albers and Marian C. Cheung and Patricia W. Wahl},
  journal={Journal of lipid research},
  year={1980},
  volume={21 7},
  pages={874-8}
}
We studied the effect of storage time and conditions on the measurement of apolipoprotein A-I and A-II by radial immunodiffusion. Purified A-I and A-II standards were stable for at least 6 months before any change in immunoreactivity was detected if stored at 4 degrees C at concentrations of 0.06-0.24 mg/ml for A-I and 0.016-0.064 mg/dl for A-II in 0.84 M tetramethylurea, 6.4 M urea, and 8 mM Tris-hydrocholoride, pH 8.0. Purified A-I (0.8-1.6 mg/ml) and A-II (0.5-1.0 mg/ml) were stable for 1… CONTINUE READING

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Purified A - I and A - II standards were stable for at least 6 months before any change in immunoreactivity was detected if stored at 4 degrees C at concentrations of 0.06 - 0.24 mg / ml for A - I and 0.016 - 0.064 mg / dl for A - II in 0.84 M tetramethylurea , 6.4 M urea , and 8 mM Tris - hydrocholoride , pH 8.0 . Purified A - I ( 0.8 - 1.6 mg / ml ) and A - II ( 0.5 - 1.0 mg / ml ) were stable for 1 year if stored at -60 degrees C in 5 mM NH4HCO3 with or without 4.2 M tetramethylurea .
Purified A - I and A - II standards were stable for at least 6 months before any change in immunoreactivity was detected if stored at 4 degrees C at concentrations of 0.06 - 0.24 mg / ml for A - I and 0.016 - 0.064 mg / dl for A - II in 0.84 M tetramethylurea , 6.4 M urea , and 8 mM Tris - hydrocholoride , pH 8.0 . Purified A - I ( 0.8 - 1.6 mg / ml ) and A - II ( 0.5 - 1.0 mg / ml ) were stable for 1 year if stored at -60 degrees C in 5 mM NH4HCO3 with or without 4.2 M tetramethylurea .
Purified A - I and A - II standards were stable for at least 6 months before any change in immunoreactivity was detected if stored at 4 degrees C at concentrations of 0.06 - 0.24 mg / ml for A - I and 0.016 - 0.064 mg / dl for A - II in 0.84 M tetramethylurea , 6.4 M urea , and 8 mM Tris - hydrocholoride , pH 8.0 . Purified A - I ( 0.8 - 1.6 mg / ml ) and A - II ( 0.5 - 1.0 mg / ml ) were stable for 1 year if stored at -60 degrees C in 5 mM NH4HCO3 with or without 4.2 M tetramethylurea .
Purified A - I and A - II standards were stable for at least 6 months before any change in immunoreactivity was detected if stored at 4 degrees C at concentrations of 0.06 - 0.24 mg / ml for A - I and 0.016 - 0.064 mg / dl for A - II in 0.84 M tetramethylurea , 6.4 M urea , and 8 mM Tris - hydrocholoride , pH 8.0 . Purified A - I ( 0.8 - 1.6 mg / ml ) and A - II ( 0.5 - 1.0 mg / ml ) were stable for 1 year if stored at -60 degrees C in 5 mM NH4HCO3 with or without 4.2 M tetramethylurea .
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