Effect of storage conditions on subpopulations of peripheral blood T lymphocytes isolated from naïve cattle and cattle infected with foot-and-mouth disease virus.

@article{Eschbaumer2016EffectOS,
  title={Effect of storage conditions on subpopulations of peripheral blood T lymphocytes isolated from na{\"i}ve cattle and cattle infected with foot-and-mouth disease virus.},
  author={Michael Eschbaumer and Carolina Stenfeldt and Juan M. Pacheco and Steven I. Rekant and Jonathan Arzt},
  journal={Veterinary clinical pathology},
  year={2016},
  volume={45 1},
  pages={110-5}
}
BACKGROUND Immunophenotyping of blood lymphocytes by flow cytometry is important in infectious disease research. In animal experiments and other longitudinal studies, the processing, prompt staining, and analysis of fresh samples is a logistical challenge and daily assay variation can confound data interpretation. OBJECTIVE This study examined the feasibility of cryopreservation and deferred analysis of bovine peripheral blood T lymphocytes from normal or infected animals. METHODS… CONTINUE READING