Effect of static seeding methods on the distribution of fibroblasts within human acellular dermis

@inproceedings{Vitacolonna2013EffectOS,
  title={Effect of static seeding methods on the distribution of fibroblasts within human acellular dermis},
  author={Mario Vitacolonna and Djeda Belharazem and Peter Hohenberger and Eric Dominic Roessner},
  booktitle={Cell and Tissue Banking},
  year={2013}
}
The purpose of this investigation was to compare different dynamic cell seeding methods regarding their seeding efficiency, homogeneity, infiltration depth and proliferation within a human acellular dermis. In addition, the growth behaviour was observed during a 12-day static in vitro culture. The dynamic methods included orbital-shaker seeding and the use of a plate centrifuge with different rotational speeds, combinations of low-pressure for matrix degassing and centrifugal seeding. Scaffolds… CONTINUE READING
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