Effect of staining procedures on the results of micronucleus assays with exfoliated oral mucosa cells.

@article{Nersesyan2006EffectOS,
  title={Effect of staining procedures on the results of micronucleus assays with exfoliated oral mucosa cells.},
  author={Armen Nersesyan and Michael Kundi and Kambis Atefie and Rolf Schulte-Hermann and Siegfried Knasm{\"u}ller},
  journal={Cancer epidemiology, biomarkers & prevention : a publication of the American Association for Cancer Research, cosponsored by the American Society of Preventive Oncology},
  year={2006},
  volume={15 10},
  pages={1835-40}
}
Micronuclei in exfoliated epithelial cells are widely used as biomarkers of cancer risk in humans. To elucidate the effect of different staining procedures on the outcome of such investigation, we conducted a study in which the micronuclei frequencies in oral mucosa cells of heavy smokers (n = 20) and nonsmokers (n = 10) were evaluated with nonspecific (Giemsa, May-Grünwald-Giemsa) and DNA-specific (4',6-diamidino-2-phenylindole, Feulgen, acridine orange) stains, whereas with Giemsa-based… CONTINUE READING