Effect of stages of lactation on the concentration of a 90-kilodalton heat shock protein in bovine mammary tissue.

@article{Watanabe1997EffectOS,
  title={Effect of stages of lactation on the concentration of a 90-kilodalton heat shock protein in bovine mammary tissue.},
  author={Atsushi Watanabe and Toru Miyamoto and Norio Katoh and Yoshihiro Takahashi},
  journal={Journal of dairy science},
  year={1997},
  volume={80 10},
  pages={2372-9}
}
From the normal mammary tissue of a Holstein cow in late lactation, a heat shock protein (90 kDa) was purified by ammonium sulfate fractionation and five-step column chromatography. From 70 g of tissue, 9.5 mg of this heat shock protein were obtained; samples had 98% purity and 19% recovery. The molecular mass of the 90-kDa heat shock protein was estimated to be 86 kDa by SDS-PAGE. Analysis of the amino-terminal amino acid sequence suggested that the protein had been purified as a mixture of… CONTINUE READING